Interactive effects of ocean acidification and nitrogen limitation on the diatom Phaeodactylum tricornutum ...

• Main Authors: Li, Wei, Gao, Kunshan, Beardall, John
• Format: Dataset
• Language: English
• Published: PANGAEA 2012
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; North Pacific; Ochrophyta; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Species; Identification; Treatment; Growth rate; Growth rate, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Carotenoids per cell; Carotenoids, standard deviation; Chlorophyll c per cell; Chlorophyll c, standard deviation; Cell size; Cell counts, percent of total; Cell counts, standard deviation; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard deviation; Effective quantum yield; Effective quantum yield, standard deviation; Cell size, standard deviation; Cell biovolume; Cell biovolume, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value; Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value, standard deviation; Carbon dioxide, reciprocal of photosynthetic affinity value; Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviation; Carbon, inorganic, dissolved; Photochemical efficiency; Photochemical efficiency, standard deviation; Respiration rate, oxygen, per cell; Respiration rate, oxygen, standard deviation; Respiration rate, oxygen, per chlorophyll a; Carbon, organic, particulate, per cell; Particulate organic carbon content per cell, standard deviation; Nitrogen, organic, particulate, per cell; Particulate organic nitrogen per cell, standard deviation; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.823110; https://doi.pangaea.de/10.1594/PANGAEA.823110

Climate change is expected to bring about alterations in the marine physical and chemical environment that will induce changes in the concentration of dissolved CO2 and in nutrient availability. These in turn are expected to affect the physiological performance of phytoplankton. In order to learn how phytoplankton respond to the predicted scenario of increased CO2 and decreased nitrogen in the surface mixed layer, we investigated the diatom Phaeodactylum tricornutum as a model organism. The cells were cultured in both low CO2 (390 µatm) and high CO2 (1000 µatm) conditions at limiting (10 µmol/L) or enriched (110 µmol/L) nitrate concentrations. Our study shows that nitrogen limitation resulted in significant decreases in cell size, pigmentation, growth rate and effective quantum yield of Phaeodactylum tricornutum, but these parameters were not affected by enhanced dissolved CO2 and lowered pH. However, increased CO2 concentration induced higher rETRmax and higher dark respiration rates and decreased the CO2 ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-20. ...