Differential acid-base regulation in various gills of the green crab Carcinus maenas: Effects of elevated environmental pCO2, supplement to: Fehsenfeld, Sandra; Weihrauch, Dirk (2013): Differential acid–base regulation in various gills of the green crab Carcinus maenas: Effects of elevated environmental pCO2. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 164(1), 54-65

• Main Authors: Fehsenfeld, Sandra, Weihrauch, Dirk
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Carcinus maenas; Containers and aquaria 20-1000 L or < 1 m**2; Gene expression incl. proteomics; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Single species; Species; Identification; Sample code/label; Treatment; pH; Haemolymph, pH; Haemolymph, pH, standard deviation; Decrease of haemolymph proton concentration; Decrease of haemolymph proton concentration, standard deviation; Ammonia excretion; Ammonia excretion, standard deviation; Decrease of haemolymph proton concentration, relative; Decrease of haemolymph proton concentration, relative, standard deviation; Ammonia excretion, relative; Ammonia excretion, relative, standard deviation; Protein name; Gene expression, fold change, relative; Gene expression, fold change, relative, standard deviation; Salinity; Temperature, water; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Coulometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.823109; https://doi.pangaea.de/10.1594/PANGAEA.823109

Euryhaline decapod crustaceans possess an efficient regulation apparatus located in the gill epithelia, providing a high adaptation potential to varying environmental abiotic conditions. Even though many studies focussed on the osmoregulatory capacity of the gills, acid-base regulatory mechanisms have obtained much less attention. In the present study, underlying principles and effects of elevated pCO2 on acid-base regulatory patterns were investigated in the green crab Carcinus maenas acclimated to diluted seawater. In gill perfusion experiments, all investigated gills 4-9 were observed to up-regulate the pH of the hemolymph by 0.1-0.2 units. Anterior gills, especially gill 4, were identified to be most efficient in the equivalent proton excretion rate. Ammonia excretion rates mirrored this pattern among gills, indicating a linkage between both processes. In specimen exposed to elevated pCO2 levels for at least 7 days, mimicking a future ocean scenario as predicted until the year 2300, hemolymph K+ and ammonia concentrations were significantly elevated, and an increased ammonia excretion rate was observed. A detailed quantitative gene expression analysis revealed that upon elevated pCO2 exposure, mRNA levels of transcripts hypothesized to be involved in ammonia and acid-base regulation (Rhesus-like protein, membrane-bound carbonic anhydrase, Na+/K+-ATPase) were affected predominantly in the non-osmoregulating anterior gills. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-20.