Ocean acidification mediates photosynthetic response to UV radiation and temperature increase in the diatom Phaeodactylum tricornutum ...

• Main Authors: Li, Yahe, Gao, Kunshan, Villafañe, Virginia E, Helbling, E Walter
• Format: Dataset
• Language: English
• Published: PANGAEA 2012
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Laboratory experiment; Laboratory strains; Light; North Pacific; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Primary production/Photosynthesis; Single species; Temperature; Species; Identification; Temperature, water; Treatment; Time in minutes; Effective quantum yield; Damage rate; Damage rate, standard deviation; Repair rate; Repair rate, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; Damage/repair ratio; Damage/repair ratio, standard deviation; Net photosynthesis rate, per cell; Net photosynthesis rate, standard deviation; Ultraviolet radiation-induced inhibition of photosynthesis; Ultraviolet radiation-induced inhibition of photosynthesis, standard deviation; Salinity; Partial pressure of carbon dioxide water at sea surface temperature wet air; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Potentiometric; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.823078; https://doi.pangaea.de/10.1594/PANGAEA.823078

Increasing atmospheric CO2 concentration is responsible for progressive ocean acidification, ocean warming as well as decreased thickness of upper mixing layer (UML), thus exposing phytoplankton cells not only to lower pH and higher temperatures but also to higher levels of solar UV radiation. In order to evaluate the combined effects of ocean acidification, UV radiation and temperature, we used the diatom Phaeodactylum tricornutum as a model organism and examined its physiological performance after grown under two CO2 concentrations (390 and 1000 µatm) for more than 20 generations. Compared to the ambient CO2 level (390 µatm), growth at the elevated CO2 concentration increased non-photochemical quenching (NPQ) of cells and partially counteracted the harm to PS II (photosystem II) caused by UV-A and UV-B. Such an effect was less pronounced under increased temperature levels. The ratio of repair to UV-B induced damage decreased with increased NPQ, reflecting induction of NPQ when repair dropped behind the ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-15. ...