(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514

• Main Authors: McKinney, Melissa A, Dietz, Rune, Sonne, Christian, De Guise, Sylvain, Skirnisson, Karl, Karlsson, Karl, Steingrimsson, Egill, Letcher, Robert J
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Event label; Sample ID; Species, common name; Species; Sample type; Area/locality; DATE/TIME; Sex; Age, comment; Proteins, total; Proteins, total, standard deviation; 7-ethoxyresorufin-O-deethylase, activity per protein mass; Standard deviation; Biological sample; Multiple investigations; Fluorescence microplate assay; International Polar Year 2007-2008 IPY; Arctic; Dietz; Beluga; Beluga whale; Beluga*; Delphinapterus leucas; International Polar Year; IPY; Pusa hispida; ringed seal; Ursus maritimus
• Online Access: https://dx.doi.org/10.1594/pangaea.816176; https://doi.pangaea.de/10.1594/PANGAEA.816176

The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic marine-feeding mammals: polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas), and ringed seal (Pusa hispida), and in laboratory rat as a mammalian model species. Greater depletion of fully brominated BDE209 (14-25% of 30pmol) and DBDPE (44-74% of 90pmol) occurred in individuals from all species relative to depletion of lower brominated PBDEs (BDEs 99,100, and 154; 0-3% of 30pmol). No evidence of simply debrominated metabolites was observed. Investigation of phenolic metabolites in rat and polar bear revealed formation of two phenolic, likely multiply debrominated, DBDPE metabolites in polar bear and one phenolic BDE154 metabolite in polar bear and rat microsomes. For BDE209 and DBDPE, observed metabolite concentrations were low to nondetectable, despite substantial parent depletion. These findings suggested possible underestimation of the ecosystem burden of total-BDE209, as well as its transformation products, and a need for research to identify and characterize the persistence and toxicity of major BDE209 metabolites. Similar cause for concern may exist regarding DBDPE, given similarities of physicochemical and environmental behavior to BDE209, current evidence of biotransformation, and increasing use of DBDPE as a replacement for BDE209. : Mean and standard deviation of interday duplicate assays (n=3 replicates/assay), except RS2 microsomal yield is of single day replicates. Sampling positions approximate. Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150