(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514
The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic mari...
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PANGAEA - Data Publisher for Earth & Environmental Science
2011
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Online Access: | https://dx.doi.org/10.1594/pangaea.816176 https://doi.pangaea.de/10.1594/PANGAEA.816176 |
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ftdatacite:10.1594/pangaea.816176 2023-05-15T15:04:55+02:00 (Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514 McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J 2011 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.816176 https://doi.pangaea.de/10.1594/PANGAEA.816176 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://dx.doi.org/10.1002/etc.535 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Event label Sample ID Species, common name Species Sample type Area/locality DATE/TIME Sex Age, comment Proteins, total Proteins, total, standard deviation 7-ethoxyresorufin-O-deethylase, activity per protein mass Standard deviation Biological sample Multiple investigations Fluorescence microplate assay International Polar Year 2007-2008 IPY Supplementary Dataset dataset Dataset 2011 ftdatacite https://doi.org/10.1594/pangaea.816176 https://doi.org/10.1002/etc.535 2021-11-05T12:55:41Z The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic marine-feeding mammals: polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas), and ringed seal (Pusa hispida), and in laboratory rat as a mammalian model species. Greater depletion of fully brominated BDE209 (14-25% of 30pmol) and DBDPE (44-74% of 90pmol) occurred in individuals from all species relative to depletion of lower brominated PBDEs (BDEs 99,100, and 154; 0-3% of 30pmol). No evidence of simply debrominated metabolites was observed. Investigation of phenolic metabolites in rat and polar bear revealed formation of two phenolic, likely multiply debrominated, DBDPE metabolites in polar bear and one phenolic BDE154 metabolite in polar bear and rat microsomes. For BDE209 and DBDPE, observed metabolite concentrations were low to nondetectable, despite substantial parent depletion. These findings suggested possible underestimation of the ecosystem burden of total-BDE209, as well as its transformation products, and a need for research to identify and characterize the persistence and toxicity of major BDE209 metabolites. Similar cause for concern may exist regarding DBDPE, given similarities of physicochemical and environmental behavior to BDE209, current evidence of biotransformation, and increasing use of DBDPE as a replacement for BDE209. : Mean and standard deviation of interday duplicate assays (n=3 replicates/assay), except RS2 microsomal yield is of single day replicates. Sampling positions approximate. Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150 Dataset Arctic Beluga Beluga whale Beluga* Delphinapterus leucas International Polar Year IPY Pusa hispida ringed seal Ursus maritimus DataCite Metadata Store (German National Library of Science and Technology) Arctic Dietz ENVELOPE(-153.167,-153.167,-86.267,-86.267) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Event label Sample ID Species, common name Species Sample type Area/locality DATE/TIME Sex Age, comment Proteins, total Proteins, total, standard deviation 7-ethoxyresorufin-O-deethylase, activity per protein mass Standard deviation Biological sample Multiple investigations Fluorescence microplate assay International Polar Year 2007-2008 IPY |
spellingShingle |
Event label Sample ID Species, common name Species Sample type Area/locality DATE/TIME Sex Age, comment Proteins, total Proteins, total, standard deviation 7-ethoxyresorufin-O-deethylase, activity per protein mass Standard deviation Biological sample Multiple investigations Fluorescence microplate assay International Polar Year 2007-2008 IPY McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J (Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514 |
topic_facet |
Event label Sample ID Species, common name Species Sample type Area/locality DATE/TIME Sex Age, comment Proteins, total Proteins, total, standard deviation 7-ethoxyresorufin-O-deethylase, activity per protein mass Standard deviation Biological sample Multiple investigations Fluorescence microplate assay International Polar Year 2007-2008 IPY |
description |
The present study assessed and compared the oxidative and reductive biotransformation of brominated flame retardants, including established polybrominated diphenyl ethers (PBDEs) and emerging decabromodiphenyl ethane (DBDPE) using an in vitro system based on liver microsomes from various arctic marine-feeding mammals: polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas), and ringed seal (Pusa hispida), and in laboratory rat as a mammalian model species. Greater depletion of fully brominated BDE209 (14-25% of 30pmol) and DBDPE (44-74% of 90pmol) occurred in individuals from all species relative to depletion of lower brominated PBDEs (BDEs 99,100, and 154; 0-3% of 30pmol). No evidence of simply debrominated metabolites was observed. Investigation of phenolic metabolites in rat and polar bear revealed formation of two phenolic, likely multiply debrominated, DBDPE metabolites in polar bear and one phenolic BDE154 metabolite in polar bear and rat microsomes. For BDE209 and DBDPE, observed metabolite concentrations were low to nondetectable, despite substantial parent depletion. These findings suggested possible underestimation of the ecosystem burden of total-BDE209, as well as its transformation products, and a need for research to identify and characterize the persistence and toxicity of major BDE209 metabolites. Similar cause for concern may exist regarding DBDPE, given similarities of physicochemical and environmental behavior to BDE209, current evidence of biotransformation, and increasing use of DBDPE as a replacement for BDE209. : Mean and standard deviation of interday duplicate assays (n=3 replicates/assay), except RS2 microsomal yield is of single day replicates. Sampling positions approximate. Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150 |
format |
Dataset |
author |
McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J |
author_facet |
McKinney, Melissa A Dietz, Rune Sonne, Christian De Guise, Sylvain Skirnisson, Karl Karlsson, Karl Steingrimsson, Egill Letcher, Robert J |
author_sort |
McKinney, Melissa A |
title |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514 |
title_short |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514 |
title_full |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514 |
title_fullStr |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514 |
title_full_unstemmed |
(Tables 1,2) Polar bear (Ursus maritimus), beluga whale (Delphinapterus leucas) and ringed seal (Pusa hispida) liver microsomal protein content and EROD activity, supplement to: McKinney, Melissa A; Dietz, Rune; Sonne, Christian; De Guise, Sylvain; Skirnisson, Karl; Karlsson, Karl; Steingrimsson, Egill; Letcher, Robert J (2011): Comparative hepatic microsomal biotransformation of selected PBDEs, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in Arctic marine-feeding mammals. Environmental Toxicology and Chemistry, 30(7), 1506-1514 |
title_sort |
(tables 1,2) polar bear (ursus maritimus), beluga whale (delphinapterus leucas) and ringed seal (pusa hispida) liver microsomal protein content and erod activity, supplement to: mckinney, melissa a; dietz, rune; sonne, christian; de guise, sylvain; skirnisson, karl; karlsson, karl; steingrimsson, egill; letcher, robert j (2011): comparative hepatic microsomal biotransformation of selected pbdes, including decabromodiphenyl ether, and decabromodiphenyl ethane flame retardants in arctic marine-feeding mammals. environmental toxicology and chemistry, 30(7), 1506-1514 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2011 |
url |
https://dx.doi.org/10.1594/pangaea.816176 https://doi.pangaea.de/10.1594/PANGAEA.816176 |
long_lat |
ENVELOPE(-153.167,-153.167,-86.267,-86.267) |
geographic |
Arctic Dietz |
geographic_facet |
Arctic Dietz |
genre |
Arctic Beluga Beluga whale Beluga* Delphinapterus leucas International Polar Year IPY Pusa hispida ringed seal Ursus maritimus |
genre_facet |
Arctic Beluga Beluga whale Beluga* Delphinapterus leucas International Polar Year IPY Pusa hispida ringed seal Ursus maritimus |
op_relation |
https://dx.doi.org/10.1002/etc.535 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.816176 https://doi.org/10.1002/etc.535 |
_version_ |
1766336659591266304 |