Enzyme activity and liver tissue PCB concentrations of chicks of northern fulmars (F. glacialis) and black-legged kittiwakes (R. tridactyla) from Kongsfjorden, supplement to: Helgason, Lisa Bjørnsdatter; Arukwe, Augustine; Gabrielsen, Geir W; Harju, Mikael; Hegseth, Marit Nøst; Heimstad, Eldbjørg S; Jørgensen, Even H; Mortensen, Anne S; Wolkers, Johannes (2010): Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1), 34-41
Arctic seabirds are exposed to a wide range of halogenated organic contaminants (HOCs). Exposure occurs mainly through food intake, and many pollutants accumulate in lipid-rich tissues. Little is known about how HOCs are biotransformed in arctic seabirds. In this study, we characterized biotransform...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
PANGAEA - Data Publisher for Earth & Environmental Science
2010
|
Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.811083 https://doi.pangaea.de/10.1594/PANGAEA.811083 |
id |
ftdatacite:10.1594/pangaea.811083 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.1594/pangaea.811083 2023-05-15T14:58:05+02:00 Enzyme activity and liver tissue PCB concentrations of chicks of northern fulmars (F. glacialis) and black-legged kittiwakes (R. tridactyla) from Kongsfjorden, supplement to: Helgason, Lisa Bjørnsdatter; Arukwe, Augustine; Gabrielsen, Geir W; Harju, Mikael; Hegseth, Marit Nøst; Heimstad, Eldbjørg S; Jørgensen, Even H; Mortensen, Anne S; Wolkers, Johannes (2010): Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1), 34-41 Helgason, Lisa Bjørnsdatter Arukwe, Augustine Gabrielsen, Geir W Harju, Mikael Hegseth, Marit Nøst Heimstad, Eldbjørg S Jørgensen, Even H Mortensen, Anne S Wolkers, Johannes 2010 application/zip https://dx.doi.org/10.1594/pangaea.811083 https://doi.pangaea.de/10.1594/PANGAEA.811083 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://dx.doi.org/10.1016/j.cbpc.2010.02.009 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Biological sample International Polar Year 2007-2008 IPY article Supplementary Collection of Datasets Collection 2010 ftdatacite https://doi.org/10.1594/pangaea.811083 https://doi.org/10.1016/j.cbpc.2010.02.009 2022-02-09T14:14:52Z Arctic seabirds are exposed to a wide range of halogenated organic contaminants (HOCs). Exposure occurs mainly through food intake, and many pollutants accumulate in lipid-rich tissues. Little is known about how HOCs are biotransformed in arctic seabirds. In this study, we characterized biotransformation enzymes in chicks of northern fulmars (Fulmarus glacialis) and black-legged kittiwakes (Rissa tridactyla) from Kongsfjorden (Svalbard, Norway). Phase I and II enzymes were analyzed at the transcriptional, translational and activity levels. For gene expression patterns, quantitative polymerase chain reactions (qPCR), using gene-sequence primers, were performed. Protein levels were analyzed using immunochemical assays of western blot with commercially available antibodies. Liver samples were analyzed for phase I and II enzyme activities using a variety of substrates including ethoxyresorufin (cytochrome (CYP)1A1/1A2), pentoxyresorufin (CYP2B), methoxyresorufin (CYP1A), benzyloxyresorufin (CYP3A), testosterone (CYP3A/CYP2B), 1-chloro-2,4-nitrobenzene (CDNB) (glutathione S-transferase (GST)) and 4-nitrophenol (uridine diphosphate glucuronyltransferase (UDPGT)). In addition, the hydroxylated (OH-) polychlorinated biphenyls (PCBs) were analyzed in the blood, liver and brain tissue, whereas the methylsulfone (MeSO2-) PCBs were analyzed in liver tissue. Results indicated the presence of phase I (CYP1A4/CYP1A5, CYP2B, and CYP3A) and phase II (GST and UDPGT) enzymes at the activity, protein and/or mRNA level in both species. Northern fulmar chicks had higher enzyme activity than black-legged kittiwake chicks. This in combination with the higher XOH-PCB to parent PCB ratios suggests that northern fulmar chicks have a different biotransformation capacity than black-legged kittiwake chicks. : Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150 Article in Journal/Newspaper Arctic Black-legged Kittiwake Fulmarus glacialis International Polar Year IPY Kongsfjord* Kongsfjorden Northern Fulmar rissa tridactyla Svalbard DataCite Metadata Store (German National Library of Science and Technology) Arctic Svalbard Norway Fulmar ENVELOPE(-46.016,-46.016,-60.616,-60.616) Harju ENVELOPE(28.733,28.733,65.567,65.567) Heimstad ENVELOPE(9.738,9.738,63.708,63.708) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Biological sample International Polar Year 2007-2008 IPY |
spellingShingle |
Biological sample International Polar Year 2007-2008 IPY Helgason, Lisa Bjørnsdatter Arukwe, Augustine Gabrielsen, Geir W Harju, Mikael Hegseth, Marit Nøst Heimstad, Eldbjørg S Jørgensen, Even H Mortensen, Anne S Wolkers, Johannes Enzyme activity and liver tissue PCB concentrations of chicks of northern fulmars (F. glacialis) and black-legged kittiwakes (R. tridactyla) from Kongsfjorden, supplement to: Helgason, Lisa Bjørnsdatter; Arukwe, Augustine; Gabrielsen, Geir W; Harju, Mikael; Hegseth, Marit Nøst; Heimstad, Eldbjørg S; Jørgensen, Even H; Mortensen, Anne S; Wolkers, Johannes (2010): Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1), 34-41 |
topic_facet |
Biological sample International Polar Year 2007-2008 IPY |
description |
Arctic seabirds are exposed to a wide range of halogenated organic contaminants (HOCs). Exposure occurs mainly through food intake, and many pollutants accumulate in lipid-rich tissues. Little is known about how HOCs are biotransformed in arctic seabirds. In this study, we characterized biotransformation enzymes in chicks of northern fulmars (Fulmarus glacialis) and black-legged kittiwakes (Rissa tridactyla) from Kongsfjorden (Svalbard, Norway). Phase I and II enzymes were analyzed at the transcriptional, translational and activity levels. For gene expression patterns, quantitative polymerase chain reactions (qPCR), using gene-sequence primers, were performed. Protein levels were analyzed using immunochemical assays of western blot with commercially available antibodies. Liver samples were analyzed for phase I and II enzyme activities using a variety of substrates including ethoxyresorufin (cytochrome (CYP)1A1/1A2), pentoxyresorufin (CYP2B), methoxyresorufin (CYP1A), benzyloxyresorufin (CYP3A), testosterone (CYP3A/CYP2B), 1-chloro-2,4-nitrobenzene (CDNB) (glutathione S-transferase (GST)) and 4-nitrophenol (uridine diphosphate glucuronyltransferase (UDPGT)). In addition, the hydroxylated (OH-) polychlorinated biphenyls (PCBs) were analyzed in the blood, liver and brain tissue, whereas the methylsulfone (MeSO2-) PCBs were analyzed in liver tissue. Results indicated the presence of phase I (CYP1A4/CYP1A5, CYP2B, and CYP3A) and phase II (GST and UDPGT) enzymes at the activity, protein and/or mRNA level in both species. Northern fulmar chicks had higher enzyme activity than black-legged kittiwake chicks. This in combination with the higher XOH-PCB to parent PCB ratios suggests that northern fulmar chicks have a different biotransformation capacity than black-legged kittiwake chicks. : Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150 |
format |
Article in Journal/Newspaper |
author |
Helgason, Lisa Bjørnsdatter Arukwe, Augustine Gabrielsen, Geir W Harju, Mikael Hegseth, Marit Nøst Heimstad, Eldbjørg S Jørgensen, Even H Mortensen, Anne S Wolkers, Johannes |
author_facet |
Helgason, Lisa Bjørnsdatter Arukwe, Augustine Gabrielsen, Geir W Harju, Mikael Hegseth, Marit Nøst Heimstad, Eldbjørg S Jørgensen, Even H Mortensen, Anne S Wolkers, Johannes |
author_sort |
Helgason, Lisa Bjørnsdatter |
title |
Enzyme activity and liver tissue PCB concentrations of chicks of northern fulmars (F. glacialis) and black-legged kittiwakes (R. tridactyla) from Kongsfjorden, supplement to: Helgason, Lisa Bjørnsdatter; Arukwe, Augustine; Gabrielsen, Geir W; Harju, Mikael; Hegseth, Marit Nøst; Heimstad, Eldbjørg S; Jørgensen, Even H; Mortensen, Anne S; Wolkers, Johannes (2010): Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1), 34-41 |
title_short |
Enzyme activity and liver tissue PCB concentrations of chicks of northern fulmars (F. glacialis) and black-legged kittiwakes (R. tridactyla) from Kongsfjorden, supplement to: Helgason, Lisa Bjørnsdatter; Arukwe, Augustine; Gabrielsen, Geir W; Harju, Mikael; Hegseth, Marit Nøst; Heimstad, Eldbjørg S; Jørgensen, Even H; Mortensen, Anne S; Wolkers, Johannes (2010): Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1), 34-41 |
title_full |
Enzyme activity and liver tissue PCB concentrations of chicks of northern fulmars (F. glacialis) and black-legged kittiwakes (R. tridactyla) from Kongsfjorden, supplement to: Helgason, Lisa Bjørnsdatter; Arukwe, Augustine; Gabrielsen, Geir W; Harju, Mikael; Hegseth, Marit Nøst; Heimstad, Eldbjørg S; Jørgensen, Even H; Mortensen, Anne S; Wolkers, Johannes (2010): Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1), 34-41 |
title_fullStr |
Enzyme activity and liver tissue PCB concentrations of chicks of northern fulmars (F. glacialis) and black-legged kittiwakes (R. tridactyla) from Kongsfjorden, supplement to: Helgason, Lisa Bjørnsdatter; Arukwe, Augustine; Gabrielsen, Geir W; Harju, Mikael; Hegseth, Marit Nøst; Heimstad, Eldbjørg S; Jørgensen, Even H; Mortensen, Anne S; Wolkers, Johannes (2010): Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1), 34-41 |
title_full_unstemmed |
Enzyme activity and liver tissue PCB concentrations of chicks of northern fulmars (F. glacialis) and black-legged kittiwakes (R. tridactyla) from Kongsfjorden, supplement to: Helgason, Lisa Bjørnsdatter; Arukwe, Augustine; Gabrielsen, Geir W; Harju, Mikael; Hegseth, Marit Nøst; Heimstad, Eldbjørg S; Jørgensen, Even H; Mortensen, Anne S; Wolkers, Johannes (2010): Biotransformation of PCBs in Arctic seabirds: Characterization of phase I and II pathways at transcriptional, translational and activity levels. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 152(1), 34-41 |
title_sort |
enzyme activity and liver tissue pcb concentrations of chicks of northern fulmars (f. glacialis) and black-legged kittiwakes (r. tridactyla) from kongsfjorden, supplement to: helgason, lisa bjørnsdatter; arukwe, augustine; gabrielsen, geir w; harju, mikael; hegseth, marit nøst; heimstad, eldbjørg s; jørgensen, even h; mortensen, anne s; wolkers, johannes (2010): biotransformation of pcbs in arctic seabirds: characterization of phase i and ii pathways at transcriptional, translational and activity levels. comparative biochemistry and physiology part c: toxicology & pharmacology, 152(1), 34-41 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2010 |
url |
https://dx.doi.org/10.1594/pangaea.811083 https://doi.pangaea.de/10.1594/PANGAEA.811083 |
long_lat |
ENVELOPE(-46.016,-46.016,-60.616,-60.616) ENVELOPE(28.733,28.733,65.567,65.567) ENVELOPE(9.738,9.738,63.708,63.708) |
geographic |
Arctic Svalbard Norway Fulmar Harju Heimstad |
geographic_facet |
Arctic Svalbard Norway Fulmar Harju Heimstad |
genre |
Arctic Black-legged Kittiwake Fulmarus glacialis International Polar Year IPY Kongsfjord* Kongsfjorden Northern Fulmar rissa tridactyla Svalbard |
genre_facet |
Arctic Black-legged Kittiwake Fulmarus glacialis International Polar Year IPY Kongsfjord* Kongsfjorden Northern Fulmar rissa tridactyla Svalbard |
op_relation |
https://dx.doi.org/10.1016/j.cbpc.2010.02.009 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.811083 https://doi.org/10.1016/j.cbpc.2010.02.009 |
_version_ |
1766330178239201280 |