qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions ...
The use of quantitative real-time polymerase chain reaction (qPCR) has become widespread due to its specificity, sensitivity and apparent ease of use. However, experimental error can be introduced at many stages during sample processing and analysis, and for this reason qPCR data are often normalise...
Main Authors: | , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
PANGAEA
2013
|
Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.810131 https://doi.pangaea.de/10.1594/PANGAEA.810131 |
id |
ftdatacite:10.1594/pangaea.810131 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.1594/pangaea.810131 2024-09-15T17:55:57+00:00 qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions ... Anderson, Kelli Elizur, Abigail 2013 application/zip https://dx.doi.org/10.1594/pangaea.810131 https://doi.pangaea.de/10.1594/PANGAEA.810131 en eng PANGAEA https://dx.doi.org/10.1186/1756-0500-5-21 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 article Collection Supplementary Publication Series of Datasets 2013 ftdatacite https://doi.org/10.1594/pangaea.81013110.1186/1756-0500-5-21 2024-08-01T10:51:52Z The use of quantitative real-time polymerase chain reaction (qPCR) has become widespread due to its specificity, sensitivity and apparent ease of use. However, experimental error can be introduced at many stages during sample processing and analysis, and for this reason qPCR data are often normalised to an internal reference gene. The present study used three freely available algorithms (GeNorm, NormFinder and BestKeeper) to assess the stability of hepatically expressed candidate reference genes (Hprt1, Tbp, Ef1a and b-tubulin) in two experiments. In the first, female Atlantic salmon (Salmo salar) broodstock of different ages were reared at either 14 or 22°C for an entire reproductive season, therefore a reference gene that does not respond to thermal challenge or reproductive condition was sought. In the second, estrogen treated juvenile salmon were maintained at the same temperatures for 14 days and a reference gene that does not respond to temperature or estrogen was required. Additionally, we performed ... : Supplement to: Anderson, Kelli; Elizur, Abigail (2012): Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures. BMC Research Notes, 5(21), 1-9 ... Article in Journal/Newspaper Atlantic salmon Salmo salar DataCite |
institution |
Open Polar |
collection |
DataCite |
op_collection_id |
ftdatacite |
language |
English |
description |
The use of quantitative real-time polymerase chain reaction (qPCR) has become widespread due to its specificity, sensitivity and apparent ease of use. However, experimental error can be introduced at many stages during sample processing and analysis, and for this reason qPCR data are often normalised to an internal reference gene. The present study used three freely available algorithms (GeNorm, NormFinder and BestKeeper) to assess the stability of hepatically expressed candidate reference genes (Hprt1, Tbp, Ef1a and b-tubulin) in two experiments. In the first, female Atlantic salmon (Salmo salar) broodstock of different ages were reared at either 14 or 22°C for an entire reproductive season, therefore a reference gene that does not respond to thermal challenge or reproductive condition was sought. In the second, estrogen treated juvenile salmon were maintained at the same temperatures for 14 days and a reference gene that does not respond to temperature or estrogen was required. Additionally, we performed ... : Supplement to: Anderson, Kelli; Elizur, Abigail (2012): Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures. BMC Research Notes, 5(21), 1-9 ... |
format |
Article in Journal/Newspaper |
author |
Anderson, Kelli Elizur, Abigail |
spellingShingle |
Anderson, Kelli Elizur, Abigail qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions ... |
author_facet |
Anderson, Kelli Elizur, Abigail |
author_sort |
Anderson, Kelli |
title |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions ... |
title_short |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions ... |
title_full |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions ... |
title_fullStr |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions ... |
title_full_unstemmed |
qPCR counts of mRNA for hepatic reference gene selection in juvenile and adult female Atlantic salmon from perturbation experiments under artificial temperature conditions ... |
title_sort |
qpcr counts of mrna for hepatic reference gene selection in juvenile and adult female atlantic salmon from perturbation experiments under artificial temperature conditions ... |
publisher |
PANGAEA |
publishDate |
2013 |
url |
https://dx.doi.org/10.1594/pangaea.810131 https://doi.pangaea.de/10.1594/PANGAEA.810131 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://dx.doi.org/10.1186/1756-0500-5-21 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_doi |
https://doi.org/10.1594/pangaea.81013110.1186/1756-0500-5-21 |
_version_ |
1810432168756772864 |