Prokaryotic growth and presence of metabolic products and contamination tracers in rocks samples from ODP Leg 187 sites ...

• Main Authors: Lysnes, Kristine, Torsvik, Terje, Thorseth, Ingunn H, Pedersen, Rolf B
• Format: Article in Journal/Newspaper
• Language: English
• Published: PANGAEA 2004
• Subjects: Drilling/drill rig; Leg187; Joides Resolution; Ocean Drilling Program ODP; Antarc*; Antarctic
• Online Access: https://dx.doi.org/10.1594/pangaea.781233; https://doi.pangaea.de/10.1594/PANGAEA.781233

The microbial population in samples of basalt drilled from the north of the Australian Antarctic Discordance (AAD) during Ocean Drilling Program Leg 187 were studied using deoxyribonucleic acid (DNA)-based methods and culturing techniques. The results showed the presence of a microbial population characteristic for the basalt environment. DNA sequence analysis revealed that microbes grouping within the Actinobacteria, green nonsulfur bacteria, the Cytophaga/Flavobacterium/Bacteroides (CFB) group, the Bacillus/Clostridium group, and the beta and gamma subclasses of the Proteobacteria were present in the basalt samples collected. The most dominant phylogenetic group, both in terms of the number of sequences retrieved and the intensities of the DNA bands obtained with the denaturing gradient gel electrophoresis analysis, was the gamma Proteobacteria. Enrichment cultures showed phylogenetic affiliation with the Actinobacteria, the CFB group, the Bacillus/Clostridium group, and the alpha, beta, gamma, and epsilon ... : Supplement to: Lysnes, Kristine; Torsvik, Terje; Thorseth, Ingunn H; Pedersen, Rolf B (2004): Microbial populations in ocean floor basalt: results from ODP Leg 187. In: Pedersen, RB; Christie, DM; Miller, DJ (eds.) Proceedings of the Ocean Drilling Program, Scientific Results, College Station, TX (Ocean Drilling Program), 187, 1-27 ...