Seawater carbonate chemistry and pteropod Limacina helicina antarctica shell dissolution during experiments, 2012, supplement to: Bednaršek, Nina; Tarling, Geraint A; Bakker, Dorothee C E; Fielding, Sophie; Cohen, Anne L; Kuzirian, Alan; McCorkle, Daniel C; Lézé, Bertrand; Montagna, Roberto (2012): Description and quantification of pteropod shell dissolution: A sensitive bioindicator of ocean acidification. Global Change Biology, 18(7), 2378-2388

• Main Authors: Bednaršek, Nina, Tarling, Geraint A, Bakker, Dorothee C E, Fielding, Sophie, Cohen, Anne L, Kuzirian, Alan, McCorkle, Daniel C, Lézé, Bertrand, Montagna, Roberto
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2012
• Subjects: Animalia; Antarctic; Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Laboratory experiment; Limacina helicina antarctica; Mollusca; Open ocean; Pelagos; Polar; Single species; Zooplankton; Experimental treatment; Time, incubation; Species; Salinity; Temperature, water; Phosphate; Silicate; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; pH; pH, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide, partial pressure, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Individuals, non-dissolving; Individuals, non-dissolving, standard-deviation; Dissolution rate; Dissolution rate, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Calcite saturation state; Titration potentiometric; Coulometric titration; Calculated using CO2SYS; Scanning electron microscope SEM; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Bakker; Bertrand; Antarc*; Antarctica; Limacina helicina; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.779926; https://doi.pangaea.de/10.1594/PANGAEA.779926

Anthropogenic ocean acidification is likely to have negative effects on marine calcifying organisms, such as shelled pteropods, by promoting dissolution of aragonite shells. Study of shell dissolution requires an accurate and sensitive method for assessing shell damage. Shell dissolution was induced through incubations in CO2 enriched seawater for between 4 and 14 days. We describe a procedure that allows the level of dissolution to be assessed and classified into three main types: Type I with partial dissolution of the prismatic layer; Type II with exposure of underlying crossed-lamellar layer, and Type III, where crossed-lamellar layer shows signs of dissolution. Levels of dissolution showed a good correspondence to the incubation conditions, with the most severe damage found in specimens held for 14 d in undersaturated condition (Ohm ~ 0.8). This methodology enables the response of small pelagic calcifiers to acidified conditions to be detected at an early stage, thus making pteropods a valuable bioindicator of future ocean acidification. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).