Seawater carbonate chemistry, nutrients, chlorophyll and diatom Proboscia alata during experiments, 2012, supplement to: Hoogstraten, Astrid; Timmermans, Klaas R; de Baar, Hein J W (2012): Morphological and physiological effects in Proboscia alata (bacillariophyceae) grown under different light and Co2 conditions of the modern Southern Ocean. Journal of Phycology, 48(3), 559-568

• Main Authors: Hoogstraten, Astrid, Timmermans, Klaas R, de Baar, Hein J W
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2012
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; North Atlantic; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Proboscia alata; Single species; Experimental treatment; Salinity; Temperature, water; Temperature, standard deviation; LightDark cycle; Radiation, photosynthetically active; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide, partial pressure, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; pH; pH, standard deviation; Replicates; Proboscia alata, length; Proboscia alata, length, standard deviation; Proboscia alata, diameter; Proboscia alata, diameter, standard deviation; Proboscia alata, volume; Proboscia alata, volume, standard deviation; Photosynthetic efficiency; Photosynthetic efficiency, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Nitrate uptake rate per cell; Nitrate uptake rate, standard deviation; Phosphate uptake rate per cell; Phosphate uptake rate, standard deviation; Silicon uptake rate per cell; Silicon uptake, standard deviation; Nitrogen/Phosphorus ratio; Nitrogen/Phosphorus ratio, standard deviation; Nitrogen/Silicon ratio; Standard deviation; Silicon/Phosphorus ratio; Silicon/Phosphorus ratio, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Potentiometric titration, VINDTA marianda; Calculated using CO2SYS; Microscopy; see references; Spectrofluorometry; Auto-analyzer, Technicon Traacs 800; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; International Polar Year 2007-2008 IPY; Ocean Acidification International Coordination Centre OA-ICC; Southern Ocean; International Polar Year; IPY; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.778472; https://doi.pangaea.de/10.1594/PANGAEA.778472

The combined effects of different light and aqueous CO2 conditions were assessed for the Southern Ocean diatom Proboscia alata(Brightwell) Sundström in laboratory experiments. Selected culture conditions (light and CO2(aq)) were representative for the natural ranges in the modern Southern Ocean. Light conditions were 40 (low) and 240 (high) µmol photons/m**2/s. The three CO2(aq) conditions ranged from 8 to 34 µmol/kg CO2(aq) (equivalent to a pCO2 from 137 to 598 µatm, respectively). Clear morphological changes were induced by these different CO2(aq) conditions. Cells in low [CO2(aq)] formed spirals, while many cells in high [CO2(aq)] disintegrated. Cell size and volume were significantly affected by the different CO2(aq) concentrations. Increasing CO2(aq) concentrations led to an increase in particulate organic carbon concentrations per cell in the high light cultures, with exactly the opposite happening in the low light cultures. However, other parameters measured were not influenced by the range of CO2(aq) treatments. This included growth rates, chlorophyll aconcentration and photosynthetic yield (FV/FM). Different light treatments had a large effect on nutrient uptake. High light conditions caused an increased nutrient uptake rate compared to cells grown in low light conditions. Light and CO2 conditions co-determined in various ways the response of P. alata to changing environmental conditions. Overall P. alata appeared to be well adapted to the natural variability in light availability and CO2(aq) concentration of the modern Southern Ocean. Nevertheless, our results showed that P. alata is susceptible to future changes in inorganic carbon concentrations in the Southern Ocean. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150