Seawater carbonate chemistry and clownfish Amphiprion percula size and otholith development during experiments, 2011, supplement to: Munday, Philip L; Hernaman, V; Dixson, Danielle L; Thorrold, Simon R (2011): Effect of ocean acidification on otolith development in larvae of a tropical marine fish. Biogeosciences, 8(6), 1631-1641

• Main Authors: Munday, Philip L, Hernaman, V, Dixson, Danielle L, Thorrold, Simon R
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Amphiprion percula; Animalia; Biomass/Abundance/Elemental composition; Calcification/Dissolution; Chordata; Containers and aquaria 20-1000 L or < 1 m**2; Growth/Morphology; Laboratory experiment; Laboratory strains; Nekton; Pelagos; Single species; South Pacific; Species; Comment; Otolith, Lithium/Calcium ratio; Otolith, Lithium/Calcium ratio, standard error; Otolith, Magnesium/Calcium ratio; Otolith, Magnesium/Calcium ratio, standard error; Otolith, Manganese/Calcium ratio; Otolith, Manganese/Calcium ratio, standard error; Otolith, Strontium/Calcium ratio; Otolith, Strontium/Calcium ratio, standard error; Otolith, Barium/Calcium ratio; Otolith, Barium/Calcium ratio, standard error; Amphiprion percula, otolith, area; Amphiprion percula, otolith, area, standard error; Amphiprion percula, otolith, length; Amphiprion percula, otolith, length, standard error; Amphiprion percula, otolith, width; Amphiprion percula, otolith, width standard error; Amphiprion percula, otolith, rectangularity; Amphiprion percula, otolith, rectangularity, standard error; Amphiprion percula, otolith, circularity; Amphiprion percula, otolith, circularity, standard error; Amphiprion percula, length; Amphiprion percula, length, standard error; pH; Salinity; Temperature, water; Temperature, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Calcite saturation state; LA-ICP-MS Thermo Finnigan Element 2; see references; Image analysis; pH meter TPS WP80; Titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.778199; https://doi.pangaea.de/10.1594/PANGAEA.778199

Calcification in many invertebrate species is predicted to decline due to ocean acidification. The potential effects of elevated CO2 and reduced carbonate saturation state on other species, such as fish, are less well understood. Fish otoliths (earbones) are composed of aragonite, and thus, might be susceptible to either the reduced availability of carbonate ions in seawater at low pH, or to changes in extracellular concentrations of bicarbonate and carbonate ions caused by acid-base regulation in fish exposed to high pCO2. We reared larvae of the clownfish Amphiprion percula from hatching to settlement at three pHNBS and pCO2 levels (control: ~pH 8.15 and 404 µatm CO2; intermediate: pH 7.8 and 1050 µatm CO2; extreme: pH 7.6 and 1721 µatm CO2) to test the possible effects of ocean acidification on otolith development. There was no effect of the intermediate treatment (pH 7.8 and 1050 µatm CO2) on otolith size, shape, symmetry between left and right otoliths, or otolith elemental chemistry, compared with controls. However, in the more extreme treatment (pH 7.6 and 1721 µatm CO2) otolith area and maximum length were larger than controls, although no other traits were significantly affected. Our results support the hypothesis that pH regulation in the otolith endolymph can lead to increased precipitation of CaCO3 in otoliths of larval fish exposed to elevated CO2, as proposed by an earlier study, however, our results also show that sensitivity varies considerably among species. Importantly, our results suggest that otolith development in clownfishes is robust to even the more pessimistic changes in ocean chemistry predicted to occur by 2100. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).