Seawater carbonate chemistry and conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and delta11B, 2012, supplement to: Heinemann, Agnes; Fietzke, Jan; Melzner, Frank; Böhm, Florian; Thomsen, Jörn; Garbe-Schönberg, Dieter; Eisenhauer, Anton (2012): Conditions of Mytilus edulis extracellular body fluids and shell composition in a pH-treatment experiment: Acid-base status, trace elements and d11B. Geochemistry, Geophysics, Geosystems, 13, Q01005

• Main Authors: Heinemann, Agnes, Fietzke, Jan, Melzner, Frank, Böhm, Florian, Thomsen, Jörn, Garbe-Schönberg, Dieter, Eisenhauer, Anton
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2012
• Subjects: Acid-base regulation; Animalia; Baltic Sea; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus edulis; Single species; Temperate; Identification; Experimental treatment; Salinity; Salinity, standard deviation; Temperature, water; Temperature, standard deviation; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide, partial pressure, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Mytilus edulis, extrapallial fluid pH; Mytilus edulis, extrapallial fluid pH, standard deviation; Replicates; Mytilus edulis, haemolymph, pH; Mytilus edulis, haemolymph, pH, standard deviation; Mytilus edulis, extrapallial fluid total carbon; Mytilus edulis, extrapallial fluid total carbon, standard deviation; Mytilus edulis, haemolymph, total dissolved inorganic carbon; Mytilus edulis, haemolymph, total dissolved inorganic carbon, standard deviation; Mytilus edulis, extrapallial fluid partial pressure of carbon dioxide; Mytilus edulis, extrapallial fluid partial pressure of carbon dioxide, standard deviation; Mytilus edulis, haemolymph, partial pressure of carbon dioxide; Mytilus edulis, haemolymph, partial pressure of carbon dioxide, standard deviation; Mytilus edulis, extrapallial fluid bicarbonate; Mytilus edulis, extrapallial fluid bicarbonate, standard deviation; Mytilus edulis, haemolymph, bicarbonate ion; Mytilus edulis, haemolymph, bicarbonate ion, standard deviation; Bicarbonate; Mytilus edulis, extrapallial fluid carbonate ion; Mytilus edulis, extrapallial fluid carbonate ion, standard deviation; Mytilus edulis, haemolymph, carbonate ion; Mytilus edulis, haemolymph, carbonate ion, standard deviation; Carbonate ion; Mytilus edulis, shell mass growth; Mytilus edulis, extrapallial fluid, boron/calcium ratio; Mytilus edulis, extrapallial fluid, boron/calcium ratio, standard deviation; Boron/Calcium ratio; Boron/Calcium ratio, standard deviation; Mytilus edulis, extrapallial fluid, strontium/calcium ratio; Mytilus edulis, extrapallial fluid, strontium/calcium ratio, standard deviation; Strontium/Calcium ratio; Strontium/Calcium ratio, standard deviation; Mytilus edulis, extrapallial fluid, magnesium/calcium ratio; Mytilus edulis, extrapallial fluid, magnesium/calcium ratio, standard deviation; Magnesium/Calcium ratio; Magnesium/Calcium ratio, standard deviation; Mytilus edulis, shell, δ11B; Mytilus edulis, shell, δ11B, standard deviation; Mytilus edulis, shell length; Mytilus edulis, shell length, standard deviation; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Conductivity meter WTW, Weilheim, Gemany; Measured; Automated CO2 analyzer CIBA-Corning 965, UK; Calculated using CO2SYS; Calculated, see references; ICP-OES, Inductively coupled plasma - optical emission spectrometry; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Thomsen; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.778194; https://doi.pangaea.de/10.1594/PANGAEA.778194

Mytilus edulis were cultured for 3 months under six different seawater pCO2 levels ranging from 380 to 4000 µatm. Specimen were taken from Kiel Fjord (Western Baltic Sea, Germany) which is a habitat with high and variable seawater pCO2 and related shifts in carbonate system speciation (e.g., low pH and low CaCO3 saturation state). Hemolymph (HL) and extrapallial fluid (EPF) samples were analyzed for pH and total dissolved inorganic carbon (CT) to calculate pCO2 and [HCO3]. A second experiment was conducted for 2 months with three different pCO2 levels (380, 1400 and 4000 µatm). Boron isotopes (delta11B) were investigated by LA-MC-ICP-MS (Laser Ablation-Multicollector-Inductively Coupled Plasma-Mass Spectrometry) in shell portions precipitated during experimental treatment time. Additionally, elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF of specimen from the second experiment were measured via ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). Extracellular pH was not significantly different in HL and EPF but systematically lower than ambient water pH. This is due to high extracellular pCO2 values, a prerequisite for metabolic CO2 excretion. No accumulation of extracellular [HCO3] was measured. Elemental ratios (B/Ca, Mg/Ca and Sr/Ca) in the EPF increased slightly with pH which is in accordance with increasing growth and calcification rates at higher seawater pH values. Boron isotope ratios were highly variable between different individuals but also within single shells. This corresponds to a high individual variability in fluid B/Ca ratios and may be due to high boron concentrations in the organic parts of the shell. The mean delta11B value shows no trend with pH but appears to represent internal pH (EPF) rather than ambient water pH. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).