Seawater carbonate chemistry and Strongylocentrotus droebachiensis larval and juvenile survival and reporductive processes, 2012, supplement to: Dupont, Sam; Dorey, Narimane; Stumpp, Meike; Melzner, Frank; Thorndyke, Mike (2013): Long-term and trans-life-cycle effects of exposure to ocean acidification in the green sea urchin Strongylocentrotus droebachiensis. Marine Biology, 160(8), 1835-1843

• Main Authors: Dupont, Sam, Dorey, Narimane, Stumpp, Meike, Melzner, Frank, Thorndyke, Mike
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Echinodermata; Laboratory experiment; Mortality/Survival; North Atlantic; Reproduction; FOS Medical biotechnology; Single species; Strongylocentrotus droebachiensis; Temperate; Identification; Experimental treatment; Comment; Species; Salinity; Temperature, water; Alkalinity, total; pH; Strongylocentrotus droebachiensis, fecundity; Strongylocentrotus droebachiensis, fecundity, standard deviation; Strongylocentrotus droebachiensis, egg, diameter; Strongylocentrotus droebachiensis, egg, diameter, standard deviation; Strongylocentrotus droebachiensis, standard deviation; Strongylocentrotus droebachiensis, larval, daily mortality; Strongylocentrotus droebachiensis, larval, daily mortality, standard deviation; Survival; Survival rate, standard deviation; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Measured after Sarazin et al 1999; Calculated; pH meter 827 Metrohm; Microscopy; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.778150; https://doi.pangaea.de/10.1594/PANGAEA.778150

Anthropogenic CO2 emissions are acidifying the world's oceans. A growing body of evidence demonstrates that ocean acidification can impact survival, growth, development and physiology of marine invertebrates. Here we tested the impact of long term (up to 16 months) and trans life-cycle (adult, embryo/larvae and juvenile) exposure to elevated pCO2 (1200 µatm, compared to control 400 µatm) on the green sea urchin Strongylocentrotus droebachiensis. Female fecundity was decreased 4.5 fold when acclimated to elevated pCO2 for 4 months during reproductive conditioning while no difference was observed in females acclimated for 16 months. Moreover, adult pre-exposure for 4 months to elevated pCO2, had a direct negative impact on subsequent larval settlement success. Five to nine times fewer offspring reached the juvenile stage in cultures using gametes collected from adults previously acclimated to high pCO2 for 4 months. However, no difference in larval survival was observed when adults were pre-exposed for 16 months to elevated pCO2. pCO2 had no direct negative impact on juvenile survival except when both larvae and juveniles were raised in elevated pCO2. These negative effects on settlement success and juvenile survival can be attributed to carry-over effects from adults to larvae and from larvae to juveniles. Our results support the contention that adult sea urchins can acclimate to moderately elevated pCO2 in a matter of a few months and that carry-over effects can exacerbate the negative impact of ocean acidification on larvae and juveniles. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).