Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1), supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590
The taxonomy of Antarctic fishes has been predominantly based on morphological characteristics rather than on genetic criteria. A typical example is the Notothenia group, which includes N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii Richardson, 1844. The Polymerase Chain Reac...
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ftdatacite:10.1594/pangaea.778124 2023-05-15T14:05:24+02:00 Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1), supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590 Machado, Cintia Shimada, Marcia Kiyoe Fragoso, Stênio Perdigao Fanta, Edith Kawall, Helena G Rodrigues, Edson Donatti, Lucélia 2012 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.778124 https://doi.pangaea.de/10.1594/PANGAEA.778124 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://dx.doi.org/10.1007/s00300-011-1152-5 https://dx.doi.org/10.1111/j.1600-0633.2006.00187.x https://dx.doi.org/10.1023/b:ebfi.0000022897.26755.da Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Characteristic Notothenia rossii Notothenia coriiceps Notothenia neglecta Dataset dataset Supplementary Dataset 2012 ftdatacite https://doi.org/10.1594/pangaea.778124 https://doi.org/10.1007/s00300-011-1152-5 https://doi.org/10.1111/j.1600-0633.2006.00187.x https://doi.org/10.1023/b:ebfi.0000022897.26755.da 2022-02-09T13:17:17Z The taxonomy of Antarctic fishes has been predominantly based on morphological characteristics rather than on genetic criteria. A typical example is the Notothenia group, which includes N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii Richardson, 1844. The Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to determine whether N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951 are different or whether they are the same species with morphological, physiological and behavioural variability. N. rossii was used as control. Mitochondrial DNA (mtDNA) was isolated from muscle specimens of N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii, which were collected in Admiralty Bay, King George Island. The DNA was used to amplify a fragment (690 base pairs) of the mitochondrial gene coding region of NADH dehydrogenase subunit 2. Further, the amplicon was digested with the following restriction enzymes: DdeI, HindIII and RsaI. The results showed a variation of the digestion pattern of the fragment amplified between N. rossii, and N. coriiceps Richardson, 1844 or N. neglecta Nybelin, 1951. However, no differences were found between N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951, on the grounds of the same genetic pattern shown by the two fish. : Figure 1 in png-format at hdl:10013/epic.38971.d007 and pdf-format at hdl:10013/epic.38971.d006. Dataset Antarc* Antarctic King George Island Notothenia rossii DataCite Metadata Store (German National Library of Science and Technology) Admiralty Bay Antarctic Edith ENVELOPE(-57.617,-57.617,-63.583,-63.583) King George Island |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Characteristic Notothenia rossii Notothenia coriiceps Notothenia neglecta |
spellingShingle |
Characteristic Notothenia rossii Notothenia coriiceps Notothenia neglecta Machado, Cintia Shimada, Marcia Kiyoe Fragoso, Stênio Perdigao Fanta, Edith Kawall, Helena G Rodrigues, Edson Donatti, Lucélia Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1), supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590 |
topic_facet |
Characteristic Notothenia rossii Notothenia coriiceps Notothenia neglecta |
description |
The taxonomy of Antarctic fishes has been predominantly based on morphological characteristics rather than on genetic criteria. A typical example is the Notothenia group, which includes N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii Richardson, 1844. The Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to determine whether N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951 are different or whether they are the same species with morphological, physiological and behavioural variability. N. rossii was used as control. Mitochondrial DNA (mtDNA) was isolated from muscle specimens of N. coriiceps Richardson, 1844, N. neglecta Nybelin, 1951 and N. rossii, which were collected in Admiralty Bay, King George Island. The DNA was used to amplify a fragment (690 base pairs) of the mitochondrial gene coding region of NADH dehydrogenase subunit 2. Further, the amplicon was digested with the following restriction enzymes: DdeI, HindIII and RsaI. The results showed a variation of the digestion pattern of the fragment amplified between N. rossii, and N. coriiceps Richardson, 1844 or N. neglecta Nybelin, 1951. However, no differences were found between N. coriiceps Richardson, 1844 and N. neglecta Nybelin, 1951, on the grounds of the same genetic pattern shown by the two fish. : Figure 1 in png-format at hdl:10013/epic.38971.d007 and pdf-format at hdl:10013/epic.38971.d006. |
format |
Dataset |
author |
Machado, Cintia Shimada, Marcia Kiyoe Fragoso, Stênio Perdigao Fanta, Edith Kawall, Helena G Rodrigues, Edson Donatti, Lucélia |
author_facet |
Machado, Cintia Shimada, Marcia Kiyoe Fragoso, Stênio Perdigao Fanta, Edith Kawall, Helena G Rodrigues, Edson Donatti, Lucélia |
author_sort |
Machado, Cintia |
title |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1), supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590 |
title_short |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1), supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590 |
title_full |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1), supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590 |
title_fullStr |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1), supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590 |
title_full_unstemmed |
Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (Table 1), supplement to: Machado, Cintia; Shimada, Marcia Kiyoe; Fragoso, Stênio Perdigao; Fanta, Edith; Kawall, Helena G; Rodrigues, Edson; Donatti, Lucélia (in prep.): Molecular differentiation by PCR-RFLP technique shows that Antarctic fishes of the genus Notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/PDI-1590 |
title_sort |
molecular differentiation by pcr-rflp technique shows that antarctic fishes of the genus notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern (table 1), supplement to: machado, cintia; shimada, marcia kiyoe; fragoso, stênio perdigao; fanta, edith; kawall, helena g; rodrigues, edson; donatti, lucélia (in prep.): molecular differentiation by pcr-rflp technique shows that antarctic fishes of the genus notothenia, claimed to be two species, are phenotypic varieties with the same genetic pattern. data submission http://issues.pangaea.de/browse/pdi-1590 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2012 |
url |
https://dx.doi.org/10.1594/pangaea.778124 https://doi.pangaea.de/10.1594/PANGAEA.778124 |
long_lat |
ENVELOPE(-57.617,-57.617,-63.583,-63.583) |
geographic |
Admiralty Bay Antarctic Edith King George Island |
geographic_facet |
Admiralty Bay Antarctic Edith King George Island |
genre |
Antarc* Antarctic King George Island Notothenia rossii |
genre_facet |
Antarc* Antarctic King George Island Notothenia rossii |
op_relation |
https://dx.doi.org/10.1007/s00300-011-1152-5 https://dx.doi.org/10.1111/j.1600-0633.2006.00187.x https://dx.doi.org/10.1023/b:ebfi.0000022897.26755.da |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.778124 https://doi.org/10.1007/s00300-011-1152-5 https://doi.org/10.1111/j.1600-0633.2006.00187.x https://doi.org/10.1023/b:ebfi.0000022897.26755.da |
_version_ |
1766277268691222528 |