Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010, supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345
Recent studies on the diazotrophic cyanobacterium Trichodesmium erythraeum(IMS101) showed that increasing CO2 partial pressure (pCO2) enhances N2 fixation and growth. Significant uncertainties remain as to the degree of the sensitivity to pCO2, its modification by other environmental factors, and un...
Main Authors: | , , , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA - Data Publisher for Earth & Environmental Science
2010
|
Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.777430 https://doi.pangaea.de/10.1594/PANGAEA.777430 |
id |
ftdatacite:10.1594/pangaea.777430 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Bacteria Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Cyanobacteria Growth/Morphology Laboratory experiment Laboratory strains Light Not applicable Other metabolic rates Pelagos Phytoplankton Primary production/Photosynthesis Respiration Single species Trichodesmium sp. Comment Salinity Temperature, water Radiation, photosynthetically active Experimental treatment Partial pressure of carbon dioxide water at sea surface temperature wet air Carbon dioxide Carbon dioxide, standard deviation Alkalinity, total Alkalinity, total, standard deviation pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Growth rate Growth rate, standard deviation Carbon, organic, particulate, per cell Particulate organic carbon content per cell, standard deviation Particulate organic nitrogen per cell Particulate organic nitrogen per cell, standard deviation Particulate organic phosphorus per cell Particulate organic phosphorus per cell, standard deviation Carbon/Nitrogen ratio Carbon/Nitrogen ratio, standard deviation Chlorophyll a per cell Chlorophyll a, standard deviation Production of particulate organic carbon per cell Particulate organic carbon, production, standard deviation Production of particulate organic nitrogen Particulate organic nitrogen production, standard deviation Gross oxygen evolution, per chlorophyll a Gross oxygen evolution, standard deviation Oxygen consumption per chlorophyll a Oxygen consumption, standard deviation Fixation of carbon in chlorophyll Fixation of carbon in chlorophyll, standard deviation Bicarbonate uptake rate in chlorophyll Bicarbonate uptake in chlorophyll, standard deviation Carbon dioxide uptake rate in chlorophyll Carbon dioxide uptake in chlorophyll, standard deviation Time in hours Nitrogen fixation rate per chlorophyll a Nitrogen fixation rate, standard deviation Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Calculated using CO2SYS Alkalinity, Gran titration Gran, 1950 pH meter Metrohm electrodes Calculated, see references Mass spectrometer ANCA-SL 20-20 Europa Scientific Calculated see references Determined by acetylene reduction assay using a gas chromatograph Calculated using seacarb after Nisumaa et al. 2010 European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS European Project on Ocean Acidification EPOCA Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Bacteria Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Cyanobacteria Growth/Morphology Laboratory experiment Laboratory strains Light Not applicable Other metabolic rates Pelagos Phytoplankton Primary production/Photosynthesis Respiration Single species Trichodesmium sp. Comment Salinity Temperature, water Radiation, photosynthetically active Experimental treatment Partial pressure of carbon dioxide water at sea surface temperature wet air Carbon dioxide Carbon dioxide, standard deviation Alkalinity, total Alkalinity, total, standard deviation pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Growth rate Growth rate, standard deviation Carbon, organic, particulate, per cell Particulate organic carbon content per cell, standard deviation Particulate organic nitrogen per cell Particulate organic nitrogen per cell, standard deviation Particulate organic phosphorus per cell Particulate organic phosphorus per cell, standard deviation Carbon/Nitrogen ratio Carbon/Nitrogen ratio, standard deviation Chlorophyll a per cell Chlorophyll a, standard deviation Production of particulate organic carbon per cell Particulate organic carbon, production, standard deviation Production of particulate organic nitrogen Particulate organic nitrogen production, standard deviation Gross oxygen evolution, per chlorophyll a Gross oxygen evolution, standard deviation Oxygen consumption per chlorophyll a Oxygen consumption, standard deviation Fixation of carbon in chlorophyll Fixation of carbon in chlorophyll, standard deviation Bicarbonate uptake rate in chlorophyll Bicarbonate uptake in chlorophyll, standard deviation Carbon dioxide uptake rate in chlorophyll Carbon dioxide uptake in chlorophyll, standard deviation Time in hours Nitrogen fixation rate per chlorophyll a Nitrogen fixation rate, standard deviation Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Calculated using CO2SYS Alkalinity, Gran titration Gran, 1950 pH meter Metrohm electrodes Calculated, see references Mass spectrometer ANCA-SL 20-20 Europa Scientific Calculated see references Determined by acetylene reduction assay using a gas chromatograph Calculated using seacarb after Nisumaa et al. 2010 European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS European Project on Ocean Acidification EPOCA Ocean Acidification International Coordination Centre OA-ICC Kranz, Sven A Levitan, Orly Richter, Klaus-Uwe Prasil, O Beran-Frank, Ilana Rost, Bjoern Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010, supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345 |
topic_facet |
Bacteria Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Cyanobacteria Growth/Morphology Laboratory experiment Laboratory strains Light Not applicable Other metabolic rates Pelagos Phytoplankton Primary production/Photosynthesis Respiration Single species Trichodesmium sp. Comment Salinity Temperature, water Radiation, photosynthetically active Experimental treatment Partial pressure of carbon dioxide water at sea surface temperature wet air Carbon dioxide Carbon dioxide, standard deviation Alkalinity, total Alkalinity, total, standard deviation pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Growth rate Growth rate, standard deviation Carbon, organic, particulate, per cell Particulate organic carbon content per cell, standard deviation Particulate organic nitrogen per cell Particulate organic nitrogen per cell, standard deviation Particulate organic phosphorus per cell Particulate organic phosphorus per cell, standard deviation Carbon/Nitrogen ratio Carbon/Nitrogen ratio, standard deviation Chlorophyll a per cell Chlorophyll a, standard deviation Production of particulate organic carbon per cell Particulate organic carbon, production, standard deviation Production of particulate organic nitrogen Particulate organic nitrogen production, standard deviation Gross oxygen evolution, per chlorophyll a Gross oxygen evolution, standard deviation Oxygen consumption per chlorophyll a Oxygen consumption, standard deviation Fixation of carbon in chlorophyll Fixation of carbon in chlorophyll, standard deviation Bicarbonate uptake rate in chlorophyll Bicarbonate uptake in chlorophyll, standard deviation Carbon dioxide uptake rate in chlorophyll Carbon dioxide uptake in chlorophyll, standard deviation Time in hours Nitrogen fixation rate per chlorophyll a Nitrogen fixation rate, standard deviation Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Calculated using CO2SYS Alkalinity, Gran titration Gran, 1950 pH meter Metrohm electrodes Calculated, see references Mass spectrometer ANCA-SL 20-20 Europa Scientific Calculated see references Determined by acetylene reduction assay using a gas chromatograph Calculated using seacarb after Nisumaa et al. 2010 European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS European Project on Ocean Acidification EPOCA Ocean Acidification International Coordination Centre OA-ICC |
description |
Recent studies on the diazotrophic cyanobacterium Trichodesmium erythraeum(IMS101) showed that increasing CO2 partial pressure (pCO2) enhances N2 fixation and growth. Significant uncertainties remain as to the degree of the sensitivity to pCO2, its modification by other environmental factors, and underlying processes causing these responses. To address these questions, we examined the responses ofTrichodesmium IMS101 grown under a matrix of low and high levels of pCO2 (150 and 900 µatm) and irradiance (50 and 200 µmol photons m-2 s-1). Growth rates as well as cellular carbon and nitrogen contents increased with increasing pCO2 and light levels in the cultures. The pCO2-dependent stimulation in organic carbon and nitrogen production was highest under low light. High pCO2 stimulated rates of N2fixation and prolonged the duration, while high light affected maximum rates only. Gross photosynthesis increased with light but did not change with pCO2. HCO3- was identified as the predominant carbon source taken up in all treatments. Inorganic carbon uptake increased with light, but only gross CO2 uptake was enhanced under high pCO2. A comparison between carbon fluxes in vivo and those derived from 13C fractionation indicates high internal carbon cycling, especially in the low-pCO2treatment under high light. Light-dependent oxygen uptake was only detected underlow pCO2 combined with high light or when low-light-acclimated cells were exposed to high light, indicating that the Mehler reaction functions also as a photoprotective mechanism in Trichodesmium. Our data confirm the pronounced pCO2 effect on N2fixation and growth in Trichodesmium and further show a strong modulation of these effects by light intensity. We attribute these responses to changes in the allocation of photosynthetic energy between carbon acquisition and the assimilation of carbon and nitrogen under elevated pCO2. These findings are supported by a complementarystudy looking at photosynthetic fluorescence parameters of photosystem II, photosynthetic unit stoichiometry (photosystem I:photosystem II), and pool sizes of key proteins in carbon and nitrogen acquisition. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). |
format |
Dataset |
author |
Kranz, Sven A Levitan, Orly Richter, Klaus-Uwe Prasil, O Beran-Frank, Ilana Rost, Bjoern |
author_facet |
Kranz, Sven A Levitan, Orly Richter, Klaus-Uwe Prasil, O Beran-Frank, Ilana Rost, Bjoern |
author_sort |
Kranz, Sven A |
title |
Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010, supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345 |
title_short |
Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010, supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345 |
title_full |
Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010, supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345 |
title_fullStr |
Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010, supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345 |
title_full_unstemmed |
Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010, supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345 |
title_sort |
seawater carbonate chemistry and combined physiological effects of co2 and light on the n2-fixing cyanobacterium trichodesmium ims101 during experiments, 2010, supplement to: kranz, sven a; levitan, orly; richter, klaus-uwe; prasil, o; beran-frank, ilana; rost, bjoern (2010): combined effects of co2 and light on the n2-fixing cyanobacterium trichodesmium ims101: physiological responses. plant physiology, 154, 334-345 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2010 |
url |
https://dx.doi.org/10.1594/pangaea.777430 https://doi.pangaea.de/10.1594/PANGAEA.777430 |
long_lat |
ENVELOPE(24.117,24.117,65.717,65.717) ENVELOPE(-60.200,-60.200,-63.733,-63.733) |
geographic |
Klaus Sven |
geographic_facet |
Klaus Sven |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1104/pp.110.159145 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.777430 https://doi.org/10.1104/pp.110.159145 |
_version_ |
1766158610536071168 |
spelling |
ftdatacite:10.1594/pangaea.777430 2023-05-15T17:51:27+02:00 Seawater carbonate chemistry and combined physiological effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101 during experiments, 2010, supplement to: Kranz, Sven A; Levitan, Orly; Richter, Klaus-Uwe; Prasil, O; Beran-Frank, Ilana; Rost, Bjoern (2010): Combined effects of CO2 and light on the N2-fixing cyanobacterium Trichodesmium IMS101: Physiological responses. Plant Physiology, 154, 334-345 Kranz, Sven A Levitan, Orly Richter, Klaus-Uwe Prasil, O Beran-Frank, Ilana Rost, Bjoern 2010 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.777430 https://doi.pangaea.de/10.1594/PANGAEA.777430 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://dx.doi.org/10.1104/pp.110.159145 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Bacteria Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Cyanobacteria Growth/Morphology Laboratory experiment Laboratory strains Light Not applicable Other metabolic rates Pelagos Phytoplankton Primary production/Photosynthesis Respiration Single species Trichodesmium sp. Comment Salinity Temperature, water Radiation, photosynthetically active Experimental treatment Partial pressure of carbon dioxide water at sea surface temperature wet air Carbon dioxide Carbon dioxide, standard deviation Alkalinity, total Alkalinity, total, standard deviation pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Growth rate Growth rate, standard deviation Carbon, organic, particulate, per cell Particulate organic carbon content per cell, standard deviation Particulate organic nitrogen per cell Particulate organic nitrogen per cell, standard deviation Particulate organic phosphorus per cell Particulate organic phosphorus per cell, standard deviation Carbon/Nitrogen ratio Carbon/Nitrogen ratio, standard deviation Chlorophyll a per cell Chlorophyll a, standard deviation Production of particulate organic carbon per cell Particulate organic carbon, production, standard deviation Production of particulate organic nitrogen Particulate organic nitrogen production, standard deviation Gross oxygen evolution, per chlorophyll a Gross oxygen evolution, standard deviation Oxygen consumption per chlorophyll a Oxygen consumption, standard deviation Fixation of carbon in chlorophyll Fixation of carbon in chlorophyll, standard deviation Bicarbonate uptake rate in chlorophyll Bicarbonate uptake in chlorophyll, standard deviation Carbon dioxide uptake rate in chlorophyll Carbon dioxide uptake in chlorophyll, standard deviation Time in hours Nitrogen fixation rate per chlorophyll a Nitrogen fixation rate, standard deviation Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Calculated using CO2SYS Alkalinity, Gran titration Gran, 1950 pH meter Metrohm electrodes Calculated, see references Mass spectrometer ANCA-SL 20-20 Europa Scientific Calculated see references Determined by acetylene reduction assay using a gas chromatograph Calculated using seacarb after Nisumaa et al. 2010 European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS European Project on Ocean Acidification EPOCA Ocean Acidification International Coordination Centre OA-ICC Dataset dataset Supplementary Dataset 2010 ftdatacite https://doi.org/10.1594/pangaea.777430 https://doi.org/10.1104/pp.110.159145 2022-02-09T12:07:01Z Recent studies on the diazotrophic cyanobacterium Trichodesmium erythraeum(IMS101) showed that increasing CO2 partial pressure (pCO2) enhances N2 fixation and growth. Significant uncertainties remain as to the degree of the sensitivity to pCO2, its modification by other environmental factors, and underlying processes causing these responses. To address these questions, we examined the responses ofTrichodesmium IMS101 grown under a matrix of low and high levels of pCO2 (150 and 900 µatm) and irradiance (50 and 200 µmol photons m-2 s-1). Growth rates as well as cellular carbon and nitrogen contents increased with increasing pCO2 and light levels in the cultures. The pCO2-dependent stimulation in organic carbon and nitrogen production was highest under low light. High pCO2 stimulated rates of N2fixation and prolonged the duration, while high light affected maximum rates only. Gross photosynthesis increased with light but did not change with pCO2. HCO3- was identified as the predominant carbon source taken up in all treatments. Inorganic carbon uptake increased with light, but only gross CO2 uptake was enhanced under high pCO2. A comparison between carbon fluxes in vivo and those derived from 13C fractionation indicates high internal carbon cycling, especially in the low-pCO2treatment under high light. Light-dependent oxygen uptake was only detected underlow pCO2 combined with high light or when low-light-acclimated cells were exposed to high light, indicating that the Mehler reaction functions also as a photoprotective mechanism in Trichodesmium. Our data confirm the pronounced pCO2 effect on N2fixation and growth in Trichodesmium and further show a strong modulation of these effects by light intensity. We attribute these responses to changes in the allocation of photosynthetic energy between carbon acquisition and the assimilation of carbon and nitrogen under elevated pCO2. These findings are supported by a complementarystudy looking at photosynthetic fluorescence parameters of photosystem II, photosynthetic unit stoichiometry (photosystem I:photosystem II), and pool sizes of key proteins in carbon and nitrogen acquisition. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) Klaus ENVELOPE(24.117,24.117,65.717,65.717) Sven ENVELOPE(-60.200,-60.200,-63.733,-63.733) |