Seawater carbonate chemistry and biological processes of Emiliania huxleyi (PML B92/11) during experiments, 2011 ...

• Main Authors: Borchard, Corinna, Borges, Alberto Vieira, Händel, Nicole, Engel, Anja
• Format: Dataset
• Language: English
• Published: PANGAEA 2011
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Macro-nutrients; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature; Experimental treatment; Sample ID; Salinity; Temperature, water; Nitrate; Phosphate; LightDark cycle; Radiation, photosynthetically active; Alkalinity, total; pH; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide; Bicarbonate ion; Carbonate ion; Calcite saturation state; Revelle factor; Experiment day; Nitrogen, inorganic, dissolved; Nitrogen, organic; Particulate organic nitrogen production; Particulate organic nitrogen per cell; Production of particulate organic nitrogen; Phosphorus, inorganic; Phosphorus, organic, particulate; Particulate organic phosphorus production; Particulate organic phosphorus per cell; Phosphorus, organic, particulate, production per cell; Carbon, organic, particulate; Particulate organic carbon production; Particulate organic carbon, per cell; Carbon, organic, particulate, production per cell; Carbon, organic, particulate/Nitrogen, organic, particulate ratio; Carbon, organic, particulate/Phosphorus, organic, particulate ratio; Nitrogen, organic, particulate/Phosphorus, organic, particulate ratio; Carbonate system computation flag; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.774802; https://doi.pangaea.de/10.1594/PANGAEA.774802

The present study investigates the combined effect of phosphorous limitation, elevated partial pressure of CO2 (pCO2) and temperature on a calcifying strain of Emiliania huxleyi (PML B92/11) by means of a fully controlled continuous culture facility. Two levels of phosphorous limitation were consecutively applied by renewal of culture media (N:P = 26) at dilution rates (D) of 0.3 d- and 0.1 d-1. CO2 and temperature conditions were 300, 550 and 900 µatm pCO2 at 14 °C and 900 µatm pCO2 at 18 °C. In general, the steady state cell density and particulate organic carbon (POC) production increased with pCO2, yielding significantly higher concentrations in cultures grown at 900 µatm pCO2 compared to 300 and 550 µatm pCO2. At 900 µatm pCO2, elevation of temperature as expected for a greenhouse ocean, further increased cell densities and POC concentrations. In contrast to POC concentration, C-quotas (pmol C cell-1) were similar at D = 0.3 d-1 in all cultures. At D = 0.1 d-1, a reduction of C-quotas by up to 15% was ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...