Seawater carbonate chemistry, calcification rate, oxygen production, maximum quantum yield, symbiont density, chlorophyll concentration and crystal width of Halimeda macroloba, Halimeda cylindracea and Marginopora vertebralis during experiments, 2011, supplement to: Sinutok, Sutinee; Hill, Ross; Doblin, Martina A; Wuhrer, Richard; Ralph, Peter J (2011): Warmer more acidic conditions cause decreased productivity and calcification in subtropical coral reef sediment-dwelling calcifiers. Limnology and Oceanography, 56(4), 1200-1212

• Main Authors: Sinutok, Sutinee, Hill, Ross, Doblin, Martina A, Wuhrer, Richard, Ralph, Peter J
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Benthos; Biomass/Abundance/Elemental composition; Calcification/Dissolution; Chlorophyta; Chromista; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Foraminifera; Growth/Morphology; Heterotrophic prokaryotes; Laboratory experiment; Macroalgae; Marginopora vertebralis; Plantae; Primary production/Photosynthesis; Single species; South Pacific; Temperature; Tropical; Identification; Experimental treatment; pH; Salinity; Temperature, water; Alkalinity, total; Alkalinity, total, standard error; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Carbon dioxide; Carbon dioxide, standard error; Carbonate ion; Carbonate ion, standard error; Bicarbonate ion; Bicarbonate ion, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Calcification rate; Calcification rate, standard error; Marginopora vertebralis, symbiont cell density; Marginopora vertebralis, symbiont density, standard error; Halimeda macroloba, chlorophyll a; Halimeda macroloba, chlorophyll a, standard error; Halimeda macroloba, chlorophyll b; Halimeda macroloba, chlorophyll b, standard error; Halimeda cylindracea, chlorophyll a; Halimeda cylindracea, chlorophyll a, standard error; Halimeda cylindracea, chlorophyll b; Halimeda cylindracea, chlorophyll b, standard error; Marginopora vertebralis, chlorophyll a; Marginopora vertebralis, chlorophyll a, standard error; Marginopora vertebralis, chlorophyll c2; Marginopora vertebralis, chlorophyll c2, standard error; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield, standard error; Oxygen production rate; Oxygen production rate, standard error; Halimeda macroloba, crystal width; Halimeda macroloba, crystal width, standard error; Halimeda cylindracea, crystal width; Halimeda cylindracea, crystal width, standard error; Marginopora vertebralis, crystal width; Marginopora vertebralis, crystal width, standard error; Carbonate system computation flag; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Measured; Autotitrator Mettler Toledo; Calculated using CO2SYS; Buoyant weighing technique Davies, 1989; Spectrophotometry; see references; Fiber-optic oxygen microsensor PSt1 with MicroTX3 transmitter Presens; Scanning electron microscope Zeiss Supra 55VP; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Heron Island; Martina; Pacific; Toledo; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.774792; https://doi.pangaea.de/10.1594/PANGAEA.774792

The effects of elevated CO2 and temperature on photosynthesis and calcification in the calcifying algae Halimeda macroloba and Halimeda cylindracea and the symbiont-bearing benthic foraminifera Marginopora vertebralis were investigated through exposure to a combination of four temperatures (28°C, 30°C, 32°C, and 34°C) and four CO2 levels (39, 61, 101, and 203 Pa; pH 8.1, 7.9, 7.7, and 7.4, respectively). Elevated CO2 caused a profound decline in photosynthetic efficiency (FV : FM), calcification, and growth in all species. After five weeks at 34°C under all CO2 levels, all species died. Chlorophyll (Chl) a and b concentration in Halimeda spp. significantly decreased in 203 Pa, 32°C and 34°C treatments, but Chl a and Chl c2 concentration in M. vertebralis was not affected by temperature alone, with significant declines in the 61, 101, and 203 Pa treatments at 28°C. Significant decreases in FV : FM in all species were found after 5 weeks of exposure to elevated CO2 (203 Pa in all temperature treatments) and temperature (32°C and 34°C in all pH treatments). The rate of oxygen production declined at 61, 101, and 203 Pa in all temperature treatments for all species. The elevated CO2 and temperature treatments greatly reduced calcification (growth and crystal size) in M. vertebralis and, to a lesser extent, in Halimeda spp. These findings indicate that 32°C and 101 Pa CO2, are the upper limits for survival of these species on Heron Island reef, and we conclude that these species will be highly vulnerable to the predicted future climate change scenarios of elevated temperature and ocean acidification. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).