Seawater carbonate chemistry, mass, length and gene expression of Sepia officinalis during experiments, 2011, supplement to: Hu, Marian Y; Tseng, Yung-Che; Stumpp, Meike; Gutowska, Magdalena A; Kiko, Rainer; Lucassen, Magnus; Melzner, Frank (2011): Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis. American Journal of Physiology, 300(5), R1100-R1114

• Main Authors: Hu, Marian Y, Tseng, Yung-Che, Stumpp, Meike, Gutowska, Magdalena A, Kiko, Rainer, Lucassen, Magnus, Melzner, Frank
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Gene expression incl. proteomics; Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; Sepia officinalis; Single species; Temperate; Species; Comment; Experimental treatment; Experiment day; Salinity; Salinity, standard deviation; Temperature, water; Temperature, standard deviation; pH; pH, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Sepia officinalis, mass; Sepia officinalis, mass, standard deviation; Sepia officinalis, length, mantle; Sepia officinalis, length, mantle, standard deviation; Sodium/Potassium adenosine triphosphatase gene expression; Sodium/Potassium adenosine triphosphatase gene expression, standard deviation; Sodium/bicarbonate cotransporter gene expression; Sodium/bicarbonate cotransporter gene expression, standard deviation; Sodium driven chlorine/bicarbonate exchanger gene expression; Sodium driven chlorine/bicarbonate exchanger gene expression, standard deviation; Carbonic anhydrase gene expression; Carbonic anhydrase gene expression, standard deviation; Adenosine triphosphate synthase gene expression; Adenosine triphosphate synthase gene expression, standard deviation; Cytochrome P-450 gene expression; Cytochrome P-450 gene expression, standard deviation; Cytochrome-c-oxydase gene expression; Cytochrome-c-oxydase gene expression, standard deviation; Octopine dehydrogenase gene expression; Octopine dehydrogenase gene expression, standard deviation; Sodium/Potassium adenosine triphosphatase maximum; Sodium/Potassium adenosine triphosphatase maximum, standard deviation; Sodium/Potassium adenosine triphosphatase relative protein concentration; Sodium/Potassium adenosine triphosphatase relative protein concentration, standard deviation; Sodium/Potassium adenosine triphosphatase relative mRNA expression; Sodium/Potassium adenosine triphosphatase relative mRNA expression, standard deviation; Sepia officinalis, perivitelline fluid, pH; Sepia officinalis, perivitelline fluid, pCO2; Gene name; Sepia officinalis, mRNA gene expression; Sepia officinalis, mRNA gene expression, standard deviation; Sepia officinalis, mRNA gene expression change; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Calcite saturation state; WTW 340i pH-analyzer and WTW SenTix 81-electrode; Calculated using CO2SYS; Potentiometric open-cell titration; Precision scale Sartorius TE64, Sartorius AG, Germany; Stereomicroscopy Leica; see references; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Marian; Sonka; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.774787; https://doi.pangaea.de/10.1594/PANGAEA.774787

The specific transporters involved in maintenance of blood pH homeostasis in cephalopod molluscs have not been identified to date. Using in situ hybridization and immuno histochemical methods, we demonstrate that Na+/K+-ATPase (soNKA), a V-type H+-ATPase (soV-HA), and Na+/HCO3- cotransporter (soNBC)...