Seawater carbonate chemistry, mass, length and gene expression of Sepia officinalis during experiments, 2011, supplement to: Hu, Marian Y; Tseng, Yung-Che; Stumpp, Meike; Gutowska, Magdalena A; Kiko, Rainer; Lucassen, Magnus; Melzner, Frank (2011): Elevated seawater pCO2 differentially affects branchial acid-base transporters over the course of development in the cephalopod Sepia officinalis. American Journal of Physiology, 300(5), R1100-R1114

• Main Authors: Hu, Marian Y, Tseng, Yung-Che, Stumpp, Meike, Gutowska, Magdalena A, Kiko, Rainer, Lucassen, Magnus, Melzner, Frank
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Gene expression incl. proteomics; Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; Sepia officinalis; Single species; Temperate; Species; Comment; Experimental treatment; Experiment day; Salinity; Salinity, standard deviation; Temperature, water; Temperature, standard deviation; pH; pH, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Sepia officinalis, mass; Sepia officinalis, mass, standard deviation; Sepia officinalis, length, mantle; Sepia officinalis, length, mantle, standard deviation; Sodium/Potassium adenosine triphosphatase gene expression; Sodium/Potassium adenosine triphosphatase gene expression, standard deviation; Sodium/bicarbonate cotransporter gene expression; Sodium/bicarbonate cotransporter gene expression, standard deviation; Sodium driven chlorine/bicarbonate exchanger gene expression; Sodium driven chlorine/bicarbonate exchanger gene expression, standard deviation; Carbonic anhydrase gene expression; Carbonic anhydrase gene expression, standard deviation; Adenosine triphosphate synthase gene expression; Adenosine triphosphate synthase gene expression, standard deviation; Cytochrome P-450 gene expression; Cytochrome P-450 gene expression, standard deviation; Cytochrome-c-oxydase gene expression; Cytochrome-c-oxydase gene expression, standard deviation; Octopine dehydrogenase gene expression; Octopine dehydrogenase gene expression, standard deviation; Sodium/Potassium adenosine triphosphatase maximum; Sodium/Potassium adenosine triphosphatase maximum, standard deviation; Sodium/Potassium adenosine triphosphatase relative protein concentration; Sodium/Potassium adenosine triphosphatase relative protein concentration, standard deviation; Sodium/Potassium adenosine triphosphatase relative mRNA expression; Sodium/Potassium adenosine triphosphatase relative mRNA expression, standard deviation; Sepia officinalis, perivitelline fluid, pH; Sepia officinalis, perivitelline fluid, pCO2; Gene name; Sepia officinalis, mRNA gene expression; Sepia officinalis, mRNA gene expression, standard deviation; Sepia officinalis, mRNA gene expression change; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Calcite saturation state; WTW 340i pH-analyzer and WTW SenTix 81-electrode; Calculated using CO2SYS; Potentiometric open-cell titration; Precision scale Sartorius TE64, Sartorius AG, Germany; Stereomicroscopy Leica; see references; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Marian; Sonka; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.774787; https://doi.pangaea.de/10.1594/PANGAEA.774787

The specific transporters involved in maintenance of blood pH homeostasis in cephalopod molluscs have not been identified to date. Using in situ hybridization and immuno histochemical methods, we demonstrate that Na+/K+-ATPase (soNKA), a V-type H+-ATPase (soV-HA), and Na+/HCO3- cotransporter (soNBC) are co-localized in NKA-rich cells in the gills of Sepia officinalis. mRNA expression patterns of these transporters and selected metabolic genes were examined in response to moderately elevated seawater pCO2 (0.16 and 0.35 kPa) over a time-course of six weeks in different ontogenetic stages. The applied CO2 concentrations are relevant for ocean acidification scenarios projected for the coming decades. We determined strong expression changes in late stage embryos and hatchlings, with one to three log2-fold reductions in soNKA, soNBCe, socCAII and COX. In contrast, no hypercapnia induced changes in mRNA expression were observed in juveniles during both short- and long-term exposure. However a transiently increased demand of ion regulatory demand was evident during the initial acclimation reaction to elevated seawater pCO2. Gill Na+/K+-ATPase activity and protein concentration were increased by approximately 15% in during short (2-11 day), but not long term (42 day) exposure. Our findings support the hypothesis that the energy budget of adult cephalopods is not significantly compromised during long-term exposure to moderate environmental hypercapnia. However, the down regulation of ion-regulatory and metabolic genes in late stage embryos, taken together with a significant reduction in somatic growth, indicates that cephalopod early life stages are challenged by elevated seawater pCO2. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).