Seawater carbonate chemistry, growth rate and Emiliania huxleyi (strain AC481) biological processes during experiments, 2010 ...

• Main Authors: de Bodt, Caroline, Van Oostende, Nicolas, Harlay, Jérôme, Sabbe, Koen, Chou, Lei
• Format: Dataset
• Language: English
• Published: PANGAEA 2010
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature; Experimental treatment; Identification; Salinity; Temperature, water; Nitrate; Phosphate; Radiation, photosynthetically active; LightDark cycle; Partial pressure of carbon dioxide water at sea surface temperature wet air; Alkalinity, total; pH; Carbon, inorganic, dissolved; Carbon dioxide; Bicarbonate ion; Calcite saturation state; Species; Chlorophyll a per cell; Carbon, organic, particulate; Carbon, inorganic, particulate; Growth rate; Particulate inorganic carbon production per cell; Particulate organic carbon production per cell; Particulate inorganic carbon/particulate organic carbon ratio; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Aragonite saturation state; Calculated using CO2SYS; Alkalinity, Gran titration Gran, 1950; pH meter Metrohm electrodes; Haemocytometer counting; Measured; Element analyser, Fisons NA 1500 N; Calculated; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.772696; https://doi.pangaea.de/10.1594/PANGAEA.772696

The impact of ocean acidification and increased water temperature on marine ecosystems, in particular those involving calcifying organisms, has been gradually recognised. We examined the individual and combined effects of increased pCO2 (180 ppmV CO2, 380 ppmV CO2 and 750 ppmV CO2 corresponding to past, present and future CO2 conditions, respectively) and temperature (13 °C and 18 °C) during the exponential growth phase of the coccolithophore E. huxleyi using batch culture experiments. We showed that cellular production rate of Particulate Organic Carbon (POC) increased from the present to the future CO2 treatments at 13 °C. A significant effect of pCO2 and of temperature on calcification was found, manifesting itself in a lower cellular production rate of Particulate Inorganic Carbon (PIC) as well as a lower PIC:POC ratio at future CO2 levels and at 18 °C. Coccosphere-sized particles showed a size reduction with both increasing temperature and CO2concentration. The influence of the different treatments on ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...