Seawater carbonate chemistry, growth rate and PIC and POC production during experiments with Emiliania huxleyi (B92/11), 2011 ...

• Main Authors: Bach, Lennart Thomas, Riebesell, Ulf, Schulz, Kai Georg
• Format: Dataset
• Language: English
• Published: PANGAEA 2011
• Subjects: Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Experimental treatment; Salinity; Temperature, water; Radiation, photosynthetically active; LightDark cycle; Fugacity of carbon dioxide in seawater, standard deviation; Carbon, inorganic, dissolved; Alkalinity, total; pH; Growth rate; Emiliania huxleyi, diameter; Carbon, organic, particulate, production per cell; Carbon, inorganic, particulate, production per cell; Chlorophyll a production per cell; Particulate inorganic carbon/particulate organic carbon ratio; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Measured; Calculated using CO2SYS; Photometry; Potentiometric open-cell titration; Titration potentiometric; Calculated; Scanning electron microscope SEM; Pigments, Turner fluorometer; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.771288; https://doi.pangaea.de/10.1594/PANGAEA.771288

The coccolithophore Emiliania huxleyi was cultured under a broad range of carbonate chemistry conditions to distinguish the effects of individual carbonate system parameters on growth, primary production, and calcification. In the first experiment, alkalinity was kept constant and the fugacity of CO2(fCO2) varied from 2 to 600 Pa (1Pa ~ 10 µatm). In the second experiment, pH was kept constant (pHfree = 8) with fCO2 varying from 4 to 370 Pa. Results of the constant-alkalinity approach revealed physiological optima for growth, calcification, and organic carbon production at fCO2 values of ~20Pa, ~40 Pa, and ~80 Pa, respectively. Comparing this with the constant-pH approach showed that growth and organic carbon production increased similarly from low to intermediate CO2 levels but started to diverge towards higher CO2 levels. In the high CO2 range, growth rates and organic carbon production decreased steadily with declining pH at constant alkalinity while remaining consistently higher at constant pH. This ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...