Seawater carbonate chemistry, nitrogen concentration and macro community analyse, 2011, supplement to: Witt, Verena; Wild, Christian; Anthony, Kenneth R N; Diaz-Pulido, Guillermo; Uthicke, Sven (2011): Effects of ocean acidification on microbial community composition of, and oxygen fluxes through, biofilms from the Great Barrier Reef. Environmental Microbiology, 13(11), 2976-2989

• Main Authors: Witt, Verena, Wild, Christian, Anthony, Kenneth R N, Diaz-Pulido, Guillermo, Uthicke, Sven
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Community composition and diversity; Entire community; Laboratory experiment; Rocky-shore community; South Pacific; Temperate; Experimental treatment; Salinity; Temperature, water; pH; pH, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Alkalinity, total; Alkalinity, total, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Aragonite saturation state; Aragonite saturation state, standard deviation; Carbon, inorganic, total; Carbon, inorganic, total, standard deviation; Carbon, organic, total; Carbon, organic, total, standard deviation; Nitrogen, total; Nitrogen, total, standard deviation; Nitrogen, inorganic; Carbon/Nitrogen ratio, standard deviation; Replicates; Experiment day; Diatoms; Algae; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calcite saturation state; Temperature logger, Onset, UA-001; pH meter HQ10-HQ20 Meters, Hach, USA; Calculated using CO2SYS; Potentiometric open-cell titration; Element analyser, THERMO NA 2500; TOC analyzer Shimadzu; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Diaz; Pacific; Sven; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.770491; https://doi.pangaea.de/10.1594/PANGAEA.770491

Rising anthropogenic CO2 emissions acidify the oceans, and cause changes to seawater carbon chemistry. Bacterial biofilm communities reflect environmental disturbances and may rapidly respond to ocean acidification. This study investigates community composition and activity responses to experimental ocean acidification in biofilms from the Australian Great Barrier Reef. Natural biofilms grown on glass slides were exposed for 11 d to four controlled pCO2 concentrations representing the following scenarios: A) pre-industrial (~300 ppm), B) present-day (~400 ppm), C) mid century (~560 ppm) and D) late century (~1140 ppm). Terminal restriction fragment length polymorphism and clone library analyses of 16S rRNA genes revealed CO2-correlated bacterial community shifts between treatments A, B and D. Observed bacterial community shifts were driven by decreases in the relative abundance of Alphaproteobacteria and increases of Flavobacteriales (Bacteroidetes) at increased CO2 concentrations, indicating pH sensitivity of specific bacterial groups. Elevated pCO2 (C + D) shifted biofilm algal communities and significantly increased C and N contents, yet O2 fluxes, measured using in light and dark incubations, remained unchanged. Our findings suggest that bacterial biofilm communities rapidly adapt and reorganize in response to high pCO2 to maintain activity such as oxygen production. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).