Seawater carbonate chemistry, POC, PIC, TPC, SPM, N, TEP and growth rate during experiments with coccolithophores Emiliania huxleyi (AC472), Calcidiscus leptoporus (AC370) and Syracosphaera pulchra (AC418) during experiments, 2011, supplement to: Fiorini, Sarah; Middelburg, Jack J; Gattuso, Jean-Pierre (2011): Effects of elevated CO2 partial pressure and temperature on the coccolithophore Syracosphaera pulchra. Aquatic Microbial Ecology, 64(3), 221-232

• Main Authors: Fiorini, Sarah, Pedrotti, Maria-Luiza, Middelburg, Jack J, Gattuso, Jean-Pierre, Kerros, Marie-Emmanuelle
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Calcidiscus leptoporus; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Mediterranean Sea; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Syracosphaera pulchra; Temperature; Species; Sample ID; Experimental treatment; Salinity; Temperature, water; Radiation, photosynthetically active; LightDark cycle; Calcite saturation state; Growth rate; Carbon, inorganic, dissolved; Alkalinity, total; Carbon dioxide; pH; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Suspended particulate matter; Production of total particulate carbon per cell; Carbon, total, particulate; Total particulate carbon per cell; Carbon, organic, particulate; Carbon, organic, particulate, per cell; Carbon, inorganic, particulate, per cell; Nitrogen, total; Nitrogen; Nitrogen per cell; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Aragonite saturation state; Transparent exopolymer particles; Transparent exopolymer particles per cell; Measured; Calculated using seacarb; Coulter Counter Beckman Coulter; Calculated, see references; Calculated; Alkalinity, Gran titration Gran, 1950; pH meter Metrohm electrodes; Mass spectrometer Thermo Electron Flash EA 1122 Analyzer; Skalar AutoAnalyser; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Coulter; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.770055; https://doi.pangaea.de/10.1594/PANGAEA.770055

The response of three coccolithophores (Emiliania huxleyi, Calcidiscus leptoporus and Syracosphaera pulchra) to elevated partial pressure (pCO2) of carbon dioxide was investigated in batch cultures. For the first time, we also report on the response of the non calcifying (haploid) life stage of these three species. The growth rate, cell size, inorganic (PIC) and organic carbon (POC) of both life stages were measured at two different pCO2 (400and 760 ppm) and their organic and inorganic carbon production calculated. The two lifestages within the same species generally exhibited a similar response to elevated pCO2, theresponse of the haploid stage being often more pronounced than that of the diploid stage. Thegrowth rate was consistently higher at higher pCO2 but the response of other processes varied among species. The calcification rate of C. leptoporus and of S. pulchra did not change at elevated pCO2 while increased in E. huxleyi. The POC production as well as the cell size of both life stages of S. pulchra and of the haploid stage of E. huxleyi markedly decreased at elevated pCO2. It remained unaltered in the diploid stage of E. huxleyi and C. leptoporus and increased in the haploid stage of the latter. The PIC:POC ratio increased in E. huxleyi and was constant in C. leptoporus and S. pulchra. These results suggest that the non-calcifying stage, is more responsive than the calcifying stage and that the most versatile genera will proliferate in a more acidic ocean rather than all coccolithophores will decline. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).