Seawater carbonate chemistry, growth rate and light sensitivity of marine diatom Chaetoceros muelleri (strain CSIRO CS-176) during experiments, 2011 ...

• Main Authors: Ihnken, Sven, Roberts, Simon, Beardall, John
• Format: Dataset
• Language: English
• Published: PANGAEA 2011
• Subjects: Bottles or small containers/Aquaria <20 L; Chaetoceros muelleri; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; South Pacific; Experimental treatment; Identification; Radiation, photosynthetically active; Salinity; Temperature, water; pH, NBS scale; Carbon, inorganic, dissolved; pH; Alkalinity, total; Growth rate; Growth rate, standard deviation; Light saturation point; Light saturation point, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Light capturing capacity; Light capturing capacity, standard devitation; Carbonate system computation flag; pH, total scale; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; pH meter Metrohm electrodes; Calculated; Calculated using CO2SYS; Spectrofluorometry; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.769749; https://doi.pangaea.de/10.1594/PANGAEA.769749

This study investigated the impact of photon flux and elevated CO2 concentrations on growth and photosynthetic electron transport on the marine diatom Chaetoceros muelleri and looked for evidence for the presence of a CO2-concentrating mechanism (CCM). pH drift experiments clearly showed that C. muelleri has the capacity to use bicarbonate to acquire inorganic carbon through one or multiple CCMs. The final pH achieved in unbuffered cultures was not changed by light intensity, even under very low photon flux, implying a low energy demand of bicarbonate use via a CCM. In short-term pH drift experiments, only treatment with the carbonic anhydrase inhibitor ethoxyzolamide (EZ) slowed down the rise in pH considerably. EZ was also the only inhibitor that altered the final pH attained, although marginally. In growth experiments, CO2 availability was manipulated by changing the pH in closed flasks at a fixed dissolved inorganic carbon (DIC) concentration. Low-light-treated samples showed lower growth rates in ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...