Seawater carbonate chemistry and echinoderm Asterias rubens biological processes during experiments, 2011, supplement to: Hernroth, Bodil; Baden, Susanne; Thorndyke, Mike; Dupont, Sam (2011): Immune suppression of the echinoderm Asterias rubens (L.) following long-term ocean acidification. Aquatic Toxicology, 103(3-4), 222-224

• Main Authors: Hernroth, Bodil, Baden, Susanne, Thorndyke, Mike, Dupont, Sam
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Animalia; Asterias rubens; Benthic animals; Benthos; Containers and aquaria 20-1000 L or < 1 m**2; Echinodermata; Immunology/Self-protection; Laboratory experiment; North Atlantic; Single species; Experimental treatment; Salinity; Temperature, water; Alkalinity, total; pH; Identification; Asterias rubens, phagocytosis; Asterias rubens, coelomocyte; Asterias rubens, 70 kilodalton heat shock protein per protein mass; Asterias rubens, mitogen activated protein kinase p38; Asterias rubens, pH, coelomic fluid; Carbon, inorganic, dissolved; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Calculated; see references; pH meter Metrohm electrodes; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.767584; https://doi.pangaea.de/10.1594/PANGAEA.767584

We compared effects of exposure to predicted near-future (2100) ocean acidification (OA; pH 7.7) and normal seawater (Control; pH 8.1) on immune and stress responses in the adult sea star Asterias rubens. Analyses were made after one week and after six months of continuous exposure. Following one week exposure to acidified water, the pH of coelomic fluid was significantly reduced. Levels of the chaperon Hsp70 were elevated while key cellular players in immunity, coelomocytes, were reduced by approximately 50%. Following long-term exposure (six months) levels of Hsp70 returned to control values, whereas immunity was further impaired, evidenced by the reduced phagocytic capacity of coelomocytes and inhibited activation of p38 MAP-kinase. Such impacts of reduced seawater pH may have serious consequences for resistance to pathogens in a future acidified ocean. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).