Seawater carbonate chemistry, fertilization rate and biological processes of sea urchin Paracentrotus lividus during experiments, 2011

• Main Authors: Moulin, Laure, Catarino, Ana Isabel, Claessens, Thomas, Dubois, Philippe
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Development; Echinodermata; Laboratory experiment; Paracentrotus lividus; Reproduction; FOS Medical biotechnology; Single species; Temperate; Site; Experimental treatment; Identification; Paracentrotus lividus, rod size; Paracentrotus lividus, larvae, pluteus; Paracentrotus lividus, cleavage rate; Fertilization success rate; Salinity; Temperature, water; pH; Alkalinity, total; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Calcite saturation state; Aragonite saturation state; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Microscopy; see references; Conductivity meter WTW, Weilheim, Gemany; Metrohm Titrando titrator; Alkalinity, Gran titration Gran, 1950; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.763905; https://doi.pangaea.de/10.1594/PANGAEA.763905

The effect of pH ranging from 8.0 to 6.8 (total scale - pHT) on fertilization, cleavage and larval development until pluteus stage was assessed in an intertidal temperate sea urchin. Gametes were obtained from adults collected in two contrasting tide pools, one showing a significant nocturnal pH decrease (lowest pHT = 7.4) and another where pH was more stable (lowest pHT = 7.8). The highest pHT at which significant effects on fertilization and cleavage were recorded was 7.6. On the contrary, larval development was only affected below pHT 7.4, a value equal or lower than that reported for several subtidal species. This suggests that sea urchins inhabiting stressful intertidal environments produce offspring that may better resist future ocean acidification. Moreover, at pHT 7.4, the fertilization rate of gametes whose progenitors came from the tide pool with higher pH decrease was significantly higher, indicating a possible acclimatization or adaptation of gametes to pH stress. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).