Seawater carbonate chemistry and biological processes during experiments with Patella vulgata, 2010, supplement to: Marchant, Hannah K; Calosi, Piero; Spicer, John I (2010): Short-term exposure to hypercapnia does not compromise feeding, acid–base balance or respiration of Patella vulgata but surprisingly is accompanied by radula damage. Journal of the Marine Biological Association of the United Kingdom, 90(7), 1379-1384

• Main Authors: Marchant, Hannah K, Calosi, Piero, Spicer, John I
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2010
• Subjects: Acid-base regulation; Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; North Atlantic; Patella vulgata; Respiration; Single species; Temperate; Experimental treatment; Identification; Carbonate system computation flag; Salinity; Temperature, water; pH; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Patella vulgata, haemolymph, pH; Patella vulgata, haemolymph, carbon dioxide; Patella vulgata, haemolymph, bicarbonate ion; Respiration rate, oxygen; Patella vulgata, weight; Patella vulgata, tooth, area, worn away; Feeding rate, relative; Calculated using seacarb after Nisumaa et al. 2010; CO2-Analyser Corning; Calculated using CO2SYS; pH meter Mettler Toledo InLab 413 SG; Henderson-Hasselback equasion Spicer et al., 1988; Closed respirometer technique Spicer & Eriksson, 2003; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Hannah; Toledo; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.763289; https://doi.pangaea.de/10.1594/PANGAEA.763289

The effect of short-term (5 days) exposure to CO2-acidified seawater (year 2100 predicted values, ocean pH = 7.6) on key aspects of the function of the intertidal common limpet Patella vulgata (Gastropoda: Patellidae) was investigated. Changes in extracellular acid-base balance were almost completely compensated by an increase in bicarbonate ions. A concomitant increase in haemolymph Ca2+ and visible shell dissolution implicated passive shell dissolution as the bicarbonate source. Analysis of the radula using SEM revealed that individuals from the hypercapnic treatment showed an increase in the number of damaged teeth and the extent to which such teeth were damaged compared with controls. As radula teeth are composed mainly of chitin, acid dissolution seems unlikely, and so the proximate cause of damage is unknown. There was no hypercapnia-related change in metabolism (O2 uptake) or feeding rate, also discounting the possibility that teeth damage was a result of a CO2-related increase in grazing. We conclude that although the limpet appears to have the physiological capacity to maintain its extracellular acid-base balance, metabolism and feeding rate over a 5 days exposure to acidified seawater, radular damage somehow incurred during this time could still compromise feeding in the longer term, in turn decreasing the top-down ecosystem control that P. vulgata exerts over rocky shore environments. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).