Seawater carbonate chemistry and biological processes of mussel Crassostrea gigas during experiments, 2011, supplement to: Lannig, Gisela; Eilers, Silke; Pörtner, Hans-Otto; Sokolova, Inna A; Bock, Christian (2010): Impact of ocean acidification on energy metabolism of oyster, Crassostrea gigas—Changes in metabolic pathways and thermal response. Marine Drugs, 8(8), 2318-2339

• Main Authors: Lannig, Gisela, Eilers, Silke, Pörtner, Hans-Otto, Sokolova, Inna A, Bock, Christian
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2010
• Subjects: Acid-base regulation; Animalia; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Crassostrea gigas; Laboratory experiment; Mollusca; North Atlantic; Respiration; Single species; Temperate; Temperature; Experimental treatment; Salinity; Salinity, standard deviation; Replicates; Temperature, water; pH; pH, standard deviation; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Crassostrea gigas, haemolymph, pH; Crassostrea gigas, haemolymph, partial pressure of carbon dioxide; Crassostrea gigas, haemolymph, partial pressure of oxygen; Crassostrea gigas, haemolymph, dissolved inorganic carbon; Crassostrea gigas, haemolymph, bicarbonate ion; Crassostrea gigas, haemolymph, calcium ion; Crassostrea gigas, haemolymph, sodium ion; Crassostrea gigas, haemolymph, potassium ion; Crassostrea gigas, alanine, wet tissue mass; Crassostrea gigas, succinate, wet tissue mass; Crassostrea gigas, glycogen, wet tissue mass; Crassostrea gigas, ATP, wet tissue mass; Condition index; Metabolic rate of oxygen, standard, normalized; Crassostrea gigas, respiration rate, oxygen, per cell; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Carbon, inorganic, dissolved; Alkalinity, total; Measured; Calculated using CO2SYS; Gas chromatography; Ion chromatography; TopSpin 2.5 Bruker Biospin GmbH, Germany; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Sokolova; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.761915; https://doi.pangaea.de/10.1594/PANGAEA.761915

Climate change with increasing temperature and ocean acidification (OA) poses risks for marine ecosystems. According to Pörtner and Farrell [1], synergistic effects of elevated temperature and CO2-induced OA on energy metabolism will narrow the thermal tolerance window of marine ectothermal animals. To test this hypothesis, we investigated the effect of an acute temperature rise on energy metabolism of the oyster, Crassostrea gigas chronically exposed to elevated CO2 levels (partial pressure of CO2 in the seawater ~0.15 kPa, seawater pH ~ 7.7). Within one month of incubation at elevated PCO2 and 15 °C hemolymph pH fell (pHe = 7.1 ± 0.2 (CO2-group) vs. 7.6 ± 0.1 (control)) and PeCO2 values in hemolymph increased (0.5 ± 0.2 kPa (CO2-group) vs. 0.2 ± 0.04 kPa (control)). Slightly but significantly elevated bicarbonate concentrations in the hemolymph of CO2-incubated oysters ([HCO-3]e = 1.8 ± 0.3 mM (CO2-group) vs. 1.3 ± 0.1 mM (control)) indicate only minimal regulation of extracellular acid-base status. At the acclimation temperature of 15 °C the OA-induced decrease in pHe did not lead to metabolic depression in oysters as standard metabolism rates (SMR) of CO2-exposed oysters were similar to controls. Upon acute warming SMR rose in both groups, but displayed a stronger increase in the CO2-incubated group. Investigation in isolated gill cells revealed a similar temperature-dependence of respiration between groups. Furthermore, the fraction of cellular energy demand for ion regulation via Na+/K+-ATPase was not affected by chronic hypercapnia or temperature. Metabolic profiling using 1H-NMR spectroscopy revealed substantial changes in some tissues following OA exposure at 15 °C. In mantle tissue alanine and ATP levels decreased significantly whereas an increase in succinate levels was observed in gill tissue. These findings suggest shifts in metabolic pathways following OA-exposure. Our study confirms that OA affects energy metabolism in oysters and suggests that climate change may affect populations of sessile coastal invertebrates such as mollusks : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).