Seawater carbonate chemistry and biological processes of Limacina helicina during experiments, 2011, supplement to: Lischka, Silke; Büdenbender, Jan; Boxhammer, Tim; Riebesell, Ulf (2011): Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences, 8(4), 919-932

• Main Authors: Lischka, Silke, Büdenbender, Jan, Boxhammer, Tim, Riebesell, Ulf
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Animalia; Arctic; Calcification/Dissolution; Containers and aquaria 20-1000 L or < 1 m**2; Growth/Morphology; Laboratory experiment; Limacina helicina; Mollusca; Mortality/Survival; Open ocean; Pelagos; Polar; Single species; Temperature; Zooplankton; Experimental treatment; Salinity; Temperature, water; Alkalinity, total; pH; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Silicon; Sample ID; Mortality; Limacina helicina, shell increment versus diameter; Limacina helicina, length; Limacina helicina, shell degradation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calcite saturation state; Metrohm Titrando titrator; pH meter Metrohm, 826 pH mobile; Continuous Flow Analysis; Calculated using CO2SYS; Stereomicroscopy Leica MZ 16 F; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.761910; https://doi.pangaea.de/10.1594/PANGAEA.761910

Due to their aragonitic shell, thecosome pteropods may be particularly vulnerable to ocean acidification driven by anthropogenic CO2 emissions. This applies specifically to species inhabiting Arctic surface waters that are projected to become temporarily and locally undersaturated with respect to aragonite as early as 2016. This study investigated the effects of rising partial pressure of CO2 (pCO2) and elevated temperature on pre-winter juveniles of the polar pteropod Limacina helicina. After a 29 day experiment in September/October 2009 at three different temperatures and under pCO2 scenarios projected for this century, mortality, shell degradation, shell diameter and shell increment were investigated. Temperature and pCO2 had a significant effect on mortality, but temperature was the overriding factor. Shell diameter, shell increment and shell degradation were significantly impacted by pCO2 but not by temperature. Mortality was 46% higher at 8 °C than at in situ temperature (3 °C), and 14% higher at 1100 ?atm than at 230 ?atm. Shell diameter and increment were reduced by 10 and 12% at 1100 ?atm and 230 ?atm, respectively, and shell degradation was 41% higher at elevated compared to ambient pCO2. We conclude that pre-winter juveniles will be negatively affected by both rising temperature and pCO2 which may result in a possible decline in abundance of the overwintering population, the basis for next year's reproduction. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).