Seawater carbonate chemistry and Celleporella hyalina biological processes during experiments, 2011, supplement to: Pistevos, Jennifer C A; Calosi, Piero; Widdicombe, Stephen; Bishop, John D D (2011): Will variation among genetic individuals influence species responses to global climate change? Oikos, 120(5), 675-689

• Main Authors: Pistevos, Jennifer C A, Calosi, Piero, Widdicombe, Stephen, Bishop, John D D
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2011
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Bryozoa; Celleporella hyalina; Coast and continental shelf; Growth/Morphology; Laboratory experiment; North Atlantic; Reproduction; FOS Medical biotechnology; Single species; Temperature; Experimental treatment; Salinity; Salinity, standard error; Temperature, water; Temperature, water, standard error; pH; Phenolics, all, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Alkalinity, total; Alkalinity, total, standard error; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Bicarbonate ion; Bicarbonate ion, standard error; Carbonate ion; Carbonate ion, standard error; Growth rate; Growth rate, standard error; Growth efficency; Growth efficency, standard error; Celleporella hyalina, colony condition; Celleporella hyalina, colony condition, standard error; Celleporella hyalina, reproductive investment; Celleporella hyalina, reproductive investment, standard error; Celleporella hyalina, gender allocation; Celleporella hyalina, gender allocation, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; pH meter Mettler Toledo, USA; CO2 analyser; Calculated using CO2SYS; Calculated, see references; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Toledo; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.758700; https://doi.pangaea.de/10.1594/PANGAEA.758700

Increased anthropogenic CO2 emissions in the last two centuries have lead to rising sea surface temperature and falling ocean pH, and it is predicted that current global trends will worsen over the next few decades. There is limited understanding of how genetic variation among individuals will influence the responses of populations and species to these changes. A microcosm system was set up to study the effects of predicted temperature and CO2 levels on the bryozoan Celleporella hyalina. In this marine species, colonies grow by the addition of male, female and feeding modular individuals (zooids) and can be physically subdivided to produce a clone of genetically identical colonies. We studied colony growth rate (the addition of zooids), reproductive investment (the ratio of sexual to feeding zooids) and sex ratio (male to female zooids) in four genetically distinct clonal lines. There was a significant effect of clone on growth rate, reproductive investment and sex ratio, with clones showing contrasting responses to the various temperature and pH combinations. Overall, decreasing pH and increasing temperature caused reduction of growth, and eventual cessation of growth was often observed at the highest temperature, especially during the latter half of the 15-day trials. Reproductive investment increased with increasing temperature and decreasing pH, varying more widely with temperature at the lowest pH. The increased production of males, a general stress response of the bryozoan, was seen upon exposure to reduced pH, but was not expressed at the highest temperature tested, presumably due to the frequent cessation of growth. Further to the significant effect of pH on the measured whole-colony parameters, observation by scanning electron microscopy revealed surface pitting of the calcified exoskeleton in colonies that were exposed to increased acidity. Studying ecologically relevant processes of growth and reproduction, we demonstrate the existence of relevant levels of variation among genetic individuals which may enable future adaptation via non-mutational natural selection to falling pH and rising temperature. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).