Seawater carbonate chemistry and Emiliania huxleyi biological processes during experiments, 2010, supplement to: Guan, WanChun; Gao, Kunshan (2010): Enhanced calcification ameliorates the negative effects of UV radiation on photosynthesis in the calcifying phytoplankter Emiliania huxleyi. Chinese Science Bulletin, 55(7), 588-593

• Main Authors: Guan, WanChun, Gao, Kunshan
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2010
• Subjects: Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Salinity; Temperature, water; LightDark cycle; Carbon, inorganic, dissolved; pH; Alkalinity, total; Growth rate; Growth rate, standard deviation; Carbon, inorganic, particulate, per cell; Particulate inorganic carbon per cell, standard deviation; Emiliania huxleyi, cells >9 µm; Emiliania huxleyi, cells 8-9 µm; Emiliania huxleyi, cells 5-7 µm; Emiliania huxleyi, cells <5 µm; Emiliania huxleyi, cell size, forward scatter; Emiliania huxleyi, cell size, forward scatter, stadard deviation; Effective quantum yield; Effective quantum yield, standard error; Ultraviolet radiation-induced inhibition of photosynthesis; Ultraviolet radiation-induced inhibition of photosynthesis, standard deviation; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Measured; Calculated; Calculated using CO2SYS; Microscopy; Flow cytometry; Pulse Amplitude Modulated fluorometer Diving-PAM, Walz; Steemann-Nielsen 1952 use of 14C for measuring org. prod. J Conseil 18117; Calculated using seacarb after Nisumaa et al. 2010; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Conseil; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.758212; https://doi.pangaea.de/10.1594/PANGAEA.758212

The calcifying phytoplankton species, coccolithophores, have their calcified coccoliths around the cells, however, their physiological roles are still unknown. Here, we hypothesized that the coccoliths may play a certain role in reducing solar UV radiation (UVR, 280-400 nm) and protect the cells from being harmed. Cells of Emiliania huxleyi with different thicknesses of the coccoliths were obtained by culturing them at different levels of dissolved inorganic carbon and their photophysiological responses to UVR were investigated. Although increased dissolved inorganic carbon decreased the specific growth rate, the increased coccolith thickness significantly ameliorated the photoinhibition of PSII photochemical efficiency caused by UVR. Increase by 91% in the coccolith thickness led to 35% increase of the PSII yield and 22% decrease of the photoinhibition of the effective quantum yield by UVR. The coccolith cover reduced more UVA (320-400 nm) than UVB (280-315 nm), leading to less inhibition per energy at the UV-A band. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).