Seawater carbonate chemistry and biological processes during experiments with the spider crab Hyas araneus in Kongsfjorden, Svalbard, 2009
With global climate change, ocean warming and acidification occur concomitantly. In this study, we tested the hypothesis that increasing CO2 levels affect the acid-base balance and reduce the activity capacity of the Arctic spider crab Hyas araneus, especially at the limits of thermal tolerance. Cra...
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Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2013
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Online Access: | https://dx.doi.org/10.1594/pangaea.755152 https://doi.pangaea.de/10.1594/PANGAEA.755152 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
English |
topic |
Date Experimental treatment Identification Salinity Temperature, water pH Carbon, inorganic, dissolved Partial pressure of carbon dioxide water at sea surface temperature wet air Hyas araneus, haemolymph, pH Hyas araneus, haemolymph, total carbon dioxide Hyas araneus, haemolymph, partial pressure of carbon dioxide Hyas araneus, haemolymph, partial pressure of carbon dioxide, standard deviation Hyas araneus, haemolymph, bicarbonate ion Hyas araneus, haemolymph, bicarbonate ion, standard deviation Hyas araneus, haemolymph, oxygen content Hyas araneus, haemolymph, partial pressure of oxygen Hyas araneus, haemolymph, partial pressure of oxygen, standard deviation Hyas araneus, haemolymph, lactate Hyas araneus, haemolymph, lactate, standard deviation Hyas araneus, righting responce Hyas araneus, righting responce, standard deviation pH meter Mettler Toledo, USA CO2-Analyser Corning Calculated using CO2SYS Calculated after Heisler 1986 Oxygen optode, Aanderaa Calculated Enzymatic test European Project on Ocean Acidification EPOCA |
spellingShingle |
Date Experimental treatment Identification Salinity Temperature, water pH Carbon, inorganic, dissolved Partial pressure of carbon dioxide water at sea surface temperature wet air Hyas araneus, haemolymph, pH Hyas araneus, haemolymph, total carbon dioxide Hyas araneus, haemolymph, partial pressure of carbon dioxide Hyas araneus, haemolymph, partial pressure of carbon dioxide, standard deviation Hyas araneus, haemolymph, bicarbonate ion Hyas araneus, haemolymph, bicarbonate ion, standard deviation Hyas araneus, haemolymph, oxygen content Hyas araneus, haemolymph, partial pressure of oxygen Hyas araneus, haemolymph, partial pressure of oxygen, standard deviation Hyas araneus, haemolymph, lactate Hyas araneus, haemolymph, lactate, standard deviation Hyas araneus, righting responce Hyas araneus, righting responce, standard deviation pH meter Mettler Toledo, USA CO2-Analyser Corning Calculated using CO2SYS Calculated after Heisler 1986 Oxygen optode, Aanderaa Calculated Enzymatic test European Project on Ocean Acidification EPOCA Zittier, Zora M C Hirse, Timo Pörtner, Hans-Otto Seawater carbonate chemistry and biological processes during experiments with the spider crab Hyas araneus in Kongsfjorden, Svalbard, 2009 |
topic_facet |
Date Experimental treatment Identification Salinity Temperature, water pH Carbon, inorganic, dissolved Partial pressure of carbon dioxide water at sea surface temperature wet air Hyas araneus, haemolymph, pH Hyas araneus, haemolymph, total carbon dioxide Hyas araneus, haemolymph, partial pressure of carbon dioxide Hyas araneus, haemolymph, partial pressure of carbon dioxide, standard deviation Hyas araneus, haemolymph, bicarbonate ion Hyas araneus, haemolymph, bicarbonate ion, standard deviation Hyas araneus, haemolymph, oxygen content Hyas araneus, haemolymph, partial pressure of oxygen Hyas araneus, haemolymph, partial pressure of oxygen, standard deviation Hyas araneus, haemolymph, lactate Hyas araneus, haemolymph, lactate, standard deviation Hyas araneus, righting responce Hyas araneus, righting responce, standard deviation pH meter Mettler Toledo, USA CO2-Analyser Corning Calculated using CO2SYS Calculated after Heisler 1986 Oxygen optode, Aanderaa Calculated Enzymatic test European Project on Ocean Acidification EPOCA |
description |
With global climate change, ocean warming and acidification occur concomitantly. In this study, we tested the hypothesis that increasing CO2 levels affect the acid-base balance and reduce the activity capacity of the Arctic spider crab Hyas araneus, especially at the limits of thermal tolerance. Crabs were acclimated to projected oceanic CO2 levels for 12 days (today: 380, towards the year 2100: 750 and 1,120 and beyond: 3,000 ?atm) and at two temperatures (1 and 4 °C). Effects of these treatments on the righting response (RR) were determined (1) at acclimation temperatures followed by (2) righting when exposed to an additional acute (15 min) heat stress at 12 °C. Prior to (resting) and after the consecutive stresses of combined righting activity and heat exposure, acid-base status and lactate contents were measured in the haemolymph. Under resting conditions, CO2 caused a decrease in haemolymph pH and an increase in oxygen partial pressure. Despite some buffering via an accumulation of bicarbonate, the extracellular acidosis remained uncompensated at 1 °C, a trend exacerbated when animals were acclimated to 4 °C. The additional combined exposure to activity and heat had only a slight effect on blood gas and acid-base status. Righting activity in all crabs incubated at 1 and 4 °C was unaffected by elevated CO2 levels or acute heat stress but was significantly reduced when both stressors acted synergistically. This impact was much stronger in the group acclimated at 1 °C where some individuals acclimated to high CO2 levels stopped responding. Lactate only accumulated in the haemolymph after combined righting and heat stress. In the group acclimated to 1 °C, lactate content was highest under normocapnia and lowest at the highest CO2 level in line with the finding that RR was largely reduced. In crabs acclimated to 4 °C, the RR was less affected by CO2 such that activity caused lactate to increase with rising CO2 levels. In line with the concept of oxygen and capacity limited thermal tolerance, all animals exposed to temperature extremes displayed a reduction in scope for performance, a trend exacerbated by increasing CO2 levels. Additionally, the differences seen between cold- and warm-acclimated H. araneus after heat stress indicate that a small shift to higher acclimation temperatures also alleviates the response to temperature extremes, indicating a shift in the thermal tolerance window which reduces susceptibility to additional CO2 exposure. : Seawater carbonate chemistry in different pCO2 condition tanks during the EPOCA Svalbard campaign, 2009 available at http://doi.pangaea.de/10.1594/PANGAEA.729174 |
format |
Dataset |
author |
Zittier, Zora M C Hirse, Timo Pörtner, Hans-Otto |
author_facet |
Zittier, Zora M C Hirse, Timo Pörtner, Hans-Otto |
author_sort |
Zittier, Zora M C |
title |
Seawater carbonate chemistry and biological processes during experiments with the spider crab Hyas araneus in Kongsfjorden, Svalbard, 2009 |
title_short |
Seawater carbonate chemistry and biological processes during experiments with the spider crab Hyas araneus in Kongsfjorden, Svalbard, 2009 |
title_full |
Seawater carbonate chemistry and biological processes during experiments with the spider crab Hyas araneus in Kongsfjorden, Svalbard, 2009 |
title_fullStr |
Seawater carbonate chemistry and biological processes during experiments with the spider crab Hyas araneus in Kongsfjorden, Svalbard, 2009 |
title_full_unstemmed |
Seawater carbonate chemistry and biological processes during experiments with the spider crab Hyas araneus in Kongsfjorden, Svalbard, 2009 |
title_sort |
seawater carbonate chemistry and biological processes during experiments with the spider crab hyas araneus in kongsfjorden, svalbard, 2009 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2013 |
url |
https://dx.doi.org/10.1594/pangaea.755152 https://doi.pangaea.de/10.1594/PANGAEA.755152 |
long_lat |
ENVELOPE(-67.317,-67.317,-73.700,-73.700) |
geographic |
Arctic Svalbard Toledo |
geographic_facet |
Arctic Svalbard Toledo |
genre |
Arctic Climate change Kongsfjord* Kongsfjorden Ocean acidification Svalbard |
genre_facet |
Arctic Climate change Kongsfjord* Kongsfjorden Ocean acidification Svalbard |
op_relation |
https://dx.doi.org/10.1594/pangaea.745083 https://dx.doi.org/10.1007/s00227-012-2073-8 https://dx.doi.org/10.1594/pangaea.821402 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.755152 https://doi.org/10.1594/pangaea.745083 https://doi.org/10.1007/s00227-012-2073-8 https://doi.org/10.1594/pangaea.821402 |
_version_ |
1766350235859156992 |
spelling |
ftdatacite:10.1594/pangaea.755152 2023-05-15T15:20:01+02:00 Seawater carbonate chemistry and biological processes during experiments with the spider crab Hyas araneus in Kongsfjorden, Svalbard, 2009 Zittier, Zora M C Hirse, Timo Pörtner, Hans-Otto 2013 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.755152 https://doi.pangaea.de/10.1594/PANGAEA.755152 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://dx.doi.org/10.1594/pangaea.745083 https://dx.doi.org/10.1007/s00227-012-2073-8 https://dx.doi.org/10.1594/pangaea.821402 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Date Experimental treatment Identification Salinity Temperature, water pH Carbon, inorganic, dissolved Partial pressure of carbon dioxide water at sea surface temperature wet air Hyas araneus, haemolymph, pH Hyas araneus, haemolymph, total carbon dioxide Hyas araneus, haemolymph, partial pressure of carbon dioxide Hyas araneus, haemolymph, partial pressure of carbon dioxide, standard deviation Hyas araneus, haemolymph, bicarbonate ion Hyas araneus, haemolymph, bicarbonate ion, standard deviation Hyas araneus, haemolymph, oxygen content Hyas araneus, haemolymph, partial pressure of oxygen Hyas araneus, haemolymph, partial pressure of oxygen, standard deviation Hyas araneus, haemolymph, lactate Hyas araneus, haemolymph, lactate, standard deviation Hyas araneus, righting responce Hyas araneus, righting responce, standard deviation pH meter Mettler Toledo, USA CO2-Analyser Corning Calculated using CO2SYS Calculated after Heisler 1986 Oxygen optode, Aanderaa Calculated Enzymatic test European Project on Ocean Acidification EPOCA dataset Dataset 2013 ftdatacite https://doi.org/10.1594/pangaea.755152 https://doi.org/10.1594/pangaea.745083 https://doi.org/10.1007/s00227-012-2073-8 https://doi.org/10.1594/pangaea.821402 2022-02-08T16:02:21Z With global climate change, ocean warming and acidification occur concomitantly. In this study, we tested the hypothesis that increasing CO2 levels affect the acid-base balance and reduce the activity capacity of the Arctic spider crab Hyas araneus, especially at the limits of thermal tolerance. Crabs were acclimated to projected oceanic CO2 levels for 12 days (today: 380, towards the year 2100: 750 and 1,120 and beyond: 3,000 ?atm) and at two temperatures (1 and 4 °C). Effects of these treatments on the righting response (RR) were determined (1) at acclimation temperatures followed by (2) righting when exposed to an additional acute (15 min) heat stress at 12 °C. Prior to (resting) and after the consecutive stresses of combined righting activity and heat exposure, acid-base status and lactate contents were measured in the haemolymph. Under resting conditions, CO2 caused a decrease in haemolymph pH and an increase in oxygen partial pressure. Despite some buffering via an accumulation of bicarbonate, the extracellular acidosis remained uncompensated at 1 °C, a trend exacerbated when animals were acclimated to 4 °C. The additional combined exposure to activity and heat had only a slight effect on blood gas and acid-base status. Righting activity in all crabs incubated at 1 and 4 °C was unaffected by elevated CO2 levels or acute heat stress but was significantly reduced when both stressors acted synergistically. This impact was much stronger in the group acclimated at 1 °C where some individuals acclimated to high CO2 levels stopped responding. Lactate only accumulated in the haemolymph after combined righting and heat stress. In the group acclimated to 1 °C, lactate content was highest under normocapnia and lowest at the highest CO2 level in line with the finding that RR was largely reduced. In crabs acclimated to 4 °C, the RR was less affected by CO2 such that activity caused lactate to increase with rising CO2 levels. In line with the concept of oxygen and capacity limited thermal tolerance, all animals exposed to temperature extremes displayed a reduction in scope for performance, a trend exacerbated by increasing CO2 levels. Additionally, the differences seen between cold- and warm-acclimated H. araneus after heat stress indicate that a small shift to higher acclimation temperatures also alleviates the response to temperature extremes, indicating a shift in the thermal tolerance window which reduces susceptibility to additional CO2 exposure. : Seawater carbonate chemistry in different pCO2 condition tanks during the EPOCA Svalbard campaign, 2009 available at http://doi.pangaea.de/10.1594/PANGAEA.729174 Dataset Arctic Climate change Kongsfjord* Kongsfjorden Ocean acidification Svalbard DataCite Metadata Store (German National Library of Science and Technology) Arctic Svalbard Toledo ENVELOPE(-67.317,-67.317,-73.700,-73.700) |