Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (2005 Bergen) and Coccolithus braarudii (RCC 1200), 2010, supplement to: Müller, Marius N; Schulz, Kai Georg; Riebesell, Ulf (2010): Effects of long-term high CO2 exposure on two species of coccolithophores. Biogeosciences, 7(3), 1109-1116

• Main Authors: Müller, Marius N, Schulz, Kai Georg, Riebesell, Ulf
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2010
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Coccolithus braarudii; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species; Experimental treatment; Species; Experiment day; Salinity; Temperature, water; LightDark cycle; Radiation, photosynthetically active; Nitrite; Phosphate; Partial pressure of carbon dioxide water at sea surface temperature wet air; pH; Growth rate; Carbon, organic/Nitrogen, total ratio; Particulate inorganic carbon vs total particulate nitrogen ratio; Particulate inorganic carbon/particulate organic carbon ratio; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Metrohm Titrando titrator; Calculated; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Bergen; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.744738; https://doi.pangaea.de/10.1594/PANGAEA.744738

The physiological performance of two coccolithophore species,Emiliania huxleyi and Coccolithus braarudii, was investigated during long-term exposure to elevated pCO2 levels. Mono-specific cultures were grown over 152 (E. huxleyi) and 65 (C. braarudii) generations while pCO2 was gradually increased to maximum levels of 1150 ?atm (E. huxleyi) and 930 ?atm (C. braarudii) and kept constant thereafter. Rates of cell growth and cell quotas of particulate organic carbon (POC), particulate inorganic carbon (PIC) and total particulate nitrogen (TPN) were determined repeatedly throughout the incubation period. Increasing pCO2 caused a decrease in cell growth rate of 9% and 29% in E. huxleyi and C. braarudii, respectively. In both species cellular PIC:TPN and PIC:POC ratios decreased in response to rising pCO2, whereas no change was observed in the POC:TPN ratios of E. huxleyi and C. braarudii. These results are consistent with those obtained in shorter-term high CO2exposure experiments following abrupt pertubations of the seawater carbonate system and indicate that for the strains tested here a gradual CO2 increase does not alleviate CO2/pH sensitivity. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).