Seawater carbonate chemistry and trace element accumulation during experiments with common cuttlefish, Sepia officinalis, 2009, supplement to: Lacoue-Labarthe, Thomas; Martin, Sophie; Oberhänsli, F; Teyssié, Jean-Louis; Markich, S; Ross, J; Bustamante, Paco (2009): Effects of increased pCO2 and temperature on trace element (Ag, Cd and Zn) bioaccumulation in the eggs of the common cuttlefish,Sepia officinalis. Biogeosciences, 6(11), 2561-2673

• Main Authors: Lacoue-Labarthe, Thomas, Martin, Sophie, Oberhänsli, F, Teyssié, Jean-Louis, Markich, S, Ross, J, Bustamante, Paco
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2009
• Subjects: Animalia; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Laboratory experiment; Mediterranean Sea; Mollusca; Nekton; Other studied parameter or process; Pelagos; Sepia officinalis; Single species; Temperate; Temperature; Experiment day; Identification; DATE/TIME; Experimental treatment; Carbonate system computation flag; Salinity; Temperature, water; pH; Alkalinity, total; Alkalinity, total, standard deviation; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Sepia officinalis, eggs, mass, fresh; Sepia officinalis, juvenile, mass, fresh; Manganese-54, activity, normalized; Cobalt-60, activity, normalized; Zinc-65, activity, normalized; Selenium-75, activity, normalized; Silver-110m, activity, normalized; Cadmium-109, activity, normalized; Caesium-134, activity, normalized; Americium-241, activity, normalized; Manganese-54, activity, total, Sepia officinalis, eggs; Cobalt-60, activity, total, Sepia officinalis, eggs; Zinc-65, activity, total, Sepia officinalis, eggs; Selenium-75, activity, total, Sepia officinalis, eggs; Silver-110m, activity, total, Sepia officinalis, eggs; Cadmium-109, activity, total, Sepia officinalis, eggs; Caesium-134, activity, total, Sepia officinalis, eggs; Americium-241, activity, total, Sepia officinalis, eggs; Manganese-54, activity, normalized, Sepia officinalis, eggs; Cobalt-60, activity, normalized, Sepia officinalis, eggs; Zinc-65, activity, normalized, Sepia officinalis, eggs; Selenium-75, activity, normalized, Sepia officinalis, eggs; Silver-110m, activity, normalized, Sepia officinalis, eggs; Cadmium-109, activity, normalized, Sepia officinalis, eggs; Caesium-134, activity, normalized, Sepia officinalis, eggs; Americium-241, activity, normalized, Sepia officinalis, eggs; Manganese-54, activity, standard, Sepia officinalis, eggs; Cobalt-60, activity, standard, Sepia officinalis, eggs; Zinc-65, activity, standard, Sepia officinalis, eggs; Selenium-75, activity, standard, Sepia officinalis, eggs; Silver-110m, activity, standard, Sepia officinalis, eggs; Cadmium-109, activity, standard, Sepia officinalis, eggs; Caesium-134, activity, standard, Sepia officinalis, eggs; Americium-241, activity, standard, Sepia officinalis, eggs; Manganese-54, load-concentration ratio; Cobalt-60, load-concentration ratio; Zinc-65, load-concentration ratio; Selenium-75, load-concentration ratio; Silver-110m, load-concentration ratio; Cadmium-109, load-concentration ratio; Caesium-134, load-concentration ratio; Americium-241, load-concentration ratio; Manganese-54, concentration factors; Cobalt-60, concentration factors; Zinc-65, concentration factors; Selenium-75, concentration factors; Silver-110m, concentration factors; Cadmium-109, concentration factors; Caesium-134, concentration factors; Americium-241, concentration factors; pH meter Metrohm, 826 pH mobile; Alkalinity, Gran titration Gran, 1950; Calculated using seacarb; Gamma-ray spectrometry; see references; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.736519; https://doi.pangaea.de/10.1594/PANGAEA.736519

Cephalopods play a key role in many marine trophic networks and constitute alternative fisheries resources, especially given the ongoing decline in finfish stocks. Along the European coast, the eggs of the cuttlefish Sepia officinalis are characterized by an increasing permeability of the eggshell during development, which leads to selective accumulation of essential and non-essential elements in the embryo. Temperature and pH are two critical factors that affect the metabolism of marine organisms in the coastal shallow waters. In this study, we investigated the effects of pH and temperature through a crossed (3?2; pH 8.1 (pCO2, 400 ppm), 7.85 (900 ppm) and 7.6 (1400 ppm) at 16 and 19°C, respectively) laboratory experiment. Seawater pH showed a strong effect on the egg weight and non-significant impact on the weight of hatchlings at the end of development implying an egg swelling process and embryo growth disturbances. The lower the seawater pH, the more 110 mAg was accumulated in the tissues of hatchlings. The 109Cd concentration factor (CF) decreased with decreasing pH and 65Zn CF reached maximal values pH 7.85, independently of temperature. Our results suggest that pH and temperature affected both the permeability properties of the eggshell and embryonic metabolism. To the best of our knowledge, this is one of the first studies on the consequences of ocean acidification and ocean warming on metal uptake in marine organisms, and our results indicate the need to further evaluate the likely ecotoxicological impact of the global change on the early-life stages of the cuttlefish.