Seawater carbonate chemistry and processes during experiments with phytoplankton Emiliania huxleyi (strain Bergen 2005), 2010 ...

• Main Authors: Barcelos e Ramos, Joana, Müller, Marius N, Riebesell, Ulf
• Format: Dataset
• Language: English
• Published: PANGAEA 2010
• Subjects: Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Description; Experimental treatment; Salinity; Temperature, water; Radiation, photosynthetically active; LightDark cycle; Phosphate; Alkalinity, total; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; pH; Bicarbonate ion; Carbonate ion; Carbon dioxide; Calcite saturation state; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Organic carbon fixation per cell per hour; Calcification rate of carbon per cell; Total carbon fixation per cell per hour; Cumulative carbon fixation per cell; Particulate inorganic carbon/particulate organic carbon ratio; Emiliania huxleyi, diameter; Maximum photochemical quantum yield of photosystem II; Determination of phosphate Murphy & Riley, 1962; Metrohm Titrando titrator; Automated segmented-flow analyzer Quaatro; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; see references; Coulter Counter Z series Beckman Coulter; PAM PhytoPAM, Phyto-ED Walz, PPAA0138; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Bergen; Coulter; Riley; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.736022; https://doi.pangaea.de/10.1594/PANGAEA.736022

The response of the coccolithophore Emiliania huxleyi to rising CO2 concentrations is well documented for acclimated cultures where cells are exposed to the CO2 treatments for several generations prior to the experiment. The exact number of generations required for acclimation to CO2-induced changes in seawater carbonate chemistry, however, is unknown. Here we show that Emiliania huxleyi's short-term response (26 h) after cultures (grown at 500 µatm) were abruptly exposed to changed CO2 concentrations (~190, 410, 800 and 1500 ?atm) is similar to that obtained with acclimated cultures under comparable conditions in earlier studies. Most importantly, from the lower CO2 levels (190 and 410 ?atm) to 750 and 1500 µatm calcification decreased and organic carbon fixation increased within the first 8 to 14 h after exposing the cultures to changes in carbonate chemistry. This suggests that Emiliania huxleyi rapidly alters the rates of essential metabolical processes in response to changes in seawater carbonate ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...