Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (PML B92/11), 2000 ...

• Main Authors: Riebesell, Ulf, Revill, Andrew T, Holdsworth, Daniel G, Volkman, J K
• Format: Dataset
• Language: English
• Published: PANGAEA 2000
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; North Atlantic; Other studied parameter or process; Pelagos; Phytoplankton; Single species; Experimental treatment; Salinity; Temperature, water; Carbonate system computation flag; pH; Carbon, inorganic, dissolved; Alkalinity, total; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Growth rate; δ13C, dissolved inorganic carbon; δ13C, carbon dioxide, aquatic; δ13C, particulate organic carbon; Isotopic fractionation, during photosynthis; Carbon per cell; Nitrogen per cell; Nitrogen/Carbon ratio; Experiment; CTD, Sea-Bird SBE 911plus; Calculated using seacarb after Nisumaa et al. 2010; pH meter, WTW, pH 3000; Coulometric titration; Calculated; Alkalinity, Gran titration Gran, 1950; Calculated, see references; Mass spectrometer Finnigan Delta-S; Mass spectrometer ANCA-SL 20-20 Europa Scientific; Calculated after Freeman & Hayes 1992; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Hayes; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.727288; https://doi.pangaea.de/10.1594/PANGAEA.727288

We have measured the stable carbon isotopic composition of bulk organic matter (POC), alkenones, sterols, fatty acids, and phytol in the coccolithophorid Emiliania huxleyi grown in dilute batch cultures over a wide range of CO2 concentrations (1.1-53.5 micromol L-1). The carbon isotope fractionation of POC (POC) varied by ca. 7 per mil and was positively correlated with aqueous CO2 concentration [CO2aq]. While this result confirms general trends observed for the same alga grown in nitrogen-limited chemostat cultures, considerable differences were obtained in absolute values of POC and in the slope of the relationship of POC with growth rate and [CO2aq]. Also, a significantly greater offset was obtained between the delta13C of alkenones and bulk organic matter in this study compared with previous work (5.4, cf. 3.8 per mil). This suggests that the magnitude of the isotope offset may depend on growth conditions. Relative to POC, individual fatty acids were depleted in 13C by 2.3 per mil to 4.1 per mil, phytol ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). ...