Seawater carbonate chemistry, shell linear extension and calcification during calcein staining and 45Ca experiments with pteropod Limacina helicina, 2009, supplement to: Comeau, Steeve; Gorsky, Gabriel; Jeffree, Ross; Teyssié, Jean-Louis; Gattuso, Jean-Pierre (2009): Impact of ocean acidification on a key Arctic pelagic mollusc (Limacina helicina). Biogeosciences, 6(9), 1877-1882

• Main Authors: Comeau, Steeve, Gorsky, Gabriel, Jeffree, Ross, Teyssié, Jean-Louis, Gattuso, Jean-Pierre
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2009
• Subjects: Animalia; Arctic; Bottles or small containers/Aquaria <20 L; Calcification/Dissolution; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Limacina helicina; Mollusca; Pelagos; Polar; Single species; Zooplankton; Event label; Experimental treatment; Experiment day; Carbonate system computation flag; Salinity; Temperature, water; Alkalinity, total; pH; Carbon, inorganic, dissolved; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Shell linear extension; Calcification rate of calcium carbonate; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Titration potentiometric; pH meter Metrohm, 826 pH mobile; Measured; **45Ca incorporation; European network of excellence for Ocean Ecosystems Analysis EUR-OCEANS; European Project on Ocean Acidification EPOCA; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.726856; https://doi.pangaea.de/10.1594/PANGAEA.726856

Thecosome pteropods (shelled pelagic molluscs) can play an important role in the food web of various ecosystems and play a key role in the cycling of carbon and carbonate. Since they harbor an aragonitic shell, they could be very sensitive to ocean acidification driven by the increase of anthropogenic CO2 emissions. The impact of changes in the carbonate chemistry was investigated on Limacina helicina, a key species of Arctic ecosystems. Pteropods were kept in culture under controlled pH conditions corresponding to pCO2 levels of 350 and 760 µatm. Calcification was estimated using a fluorochrome and the radioisotope 45Ca. It exhibits a 28 % decrease at the pH value expected for 2100 compared to the present pH value. This result supports the concern for the future of pteropods in a high-CO2 world, as well as of those species dependent upon them as a food resource. A decline of their populations would likely cause dramatic changes to the structure, function and services of polar ecosystems. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).