Stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in Antarctica
Many types of animal tissues are increasingly being used for stable isotope analysis, with their application dependent on the time frame they reflect and their availability for collection. Here, we investigated the isotopic values (δ13C and δ15N) of four tissues (feather, skin, toenail, and bone) co...
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U.S. Antarctic Program (USAP) Data Center
2020
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ftdatacite:10.15784/601382 2023-05-15T13:30:27+02:00 Stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in Antarctica Ciriani, Yanina Emslie, Steven 2020 https://dx.doi.org/10.15784/601382 https://www.usap-dc.org/view/dataset/601382 en eng U.S. Antarctic Program (USAP) Data Center Creative Commons Attribution-NonCommercial-Share Alike 3.0 United States [CC BY-NC-SA 3.0] https://creativecommons.org/licenses/by-nc-sa/3.0/us/ CC-BY-NC-SA Delta 13C Delta 15N Opportunistic Sampling Penguins Pygoscelis Penguins Dietary Shifts Biology Cryosphere Antarctica dataset Dataset 2020 ftdatacite https://doi.org/10.15784/601382 2021-11-05T12:55:41Z Many types of animal tissues are increasingly being used for stable isotope analysis, with their application dependent on the time frame they reflect and their availability for collection. Here, we investigated the isotopic values (δ13C and δ15N) of four tissues (feather, skin, toenail, and bone) collected from fledgling-period chick carcasses of three species of pygoscelid penguins to compare the variability and accuracy of the data among tissues. Samples were collected at 25 de Mayo/King George Island during the 2017/18 austral summer. Chick carcasses are commonly found at active penguin colonies, and “opportunistic sampling” can easily be performed without disturbing nesting penguins. A total of 25–36 carcasses per species were sampled at active colonies of Adélie Pygoscelis adeliae, Gentoo P. papua, and Chinstrap P. antarcticus penguins. A linear mixed model showed that δ13C values varied significantly between tissues, presumably due to tissue-specific isotopic discrimination. In contrast, the only tissue with significantly different δ15N values was toenail. Stable isotope data revealed dietary differences among species, with Gentoo Penguins having higher average isotopic values in tissues compared to Adélie and Chinstrap penguins. In addition, Chinstrap Penguins showed a consistent, but not statistically significant, trend in having higher δ13C values compared to Adélie Penguins. Gentoo Penguins displayed the highest isotopic variability of any species for all tissues. Isotopic composition was most variable in skin in all three species making skin the least reliable tissue for isotope analysis, whereas isotopic values were least variable in toenails. Comparison of isotopic values between two bones (tibiotarsus and coracoid) showed no significant differences in isotopic values, indicating that when the same bone is not available for sampling from carcasses, sampling of any major skeletal element is likely to provide a meaningful comparison. These results allow for more informed opportunistic sampling to accurately estimate and compare penguin diet among species and between ancient and active colonies. Dataset Antarc* Antarctica antarcticus King George Island Pygoscelis adeliae DataCite Metadata Store (German National Library of Science and Technology) Austral King George Island 25 de Mayo ENVELOPE(-58.000,-58.000,-62.083,-62.083) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Delta 13C Delta 15N Opportunistic Sampling Penguins Pygoscelis Penguins Dietary Shifts Biology Cryosphere Antarctica |
spellingShingle |
Delta 13C Delta 15N Opportunistic Sampling Penguins Pygoscelis Penguins Dietary Shifts Biology Cryosphere Antarctica Ciriani, Yanina Emslie, Steven Stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in Antarctica |
topic_facet |
Delta 13C Delta 15N Opportunistic Sampling Penguins Pygoscelis Penguins Dietary Shifts Biology Cryosphere Antarctica |
description |
Many types of animal tissues are increasingly being used for stable isotope analysis, with their application dependent on the time frame they reflect and their availability for collection. Here, we investigated the isotopic values (δ13C and δ15N) of four tissues (feather, skin, toenail, and bone) collected from fledgling-period chick carcasses of three species of pygoscelid penguins to compare the variability and accuracy of the data among tissues. Samples were collected at 25 de Mayo/King George Island during the 2017/18 austral summer. Chick carcasses are commonly found at active penguin colonies, and “opportunistic sampling” can easily be performed without disturbing nesting penguins. A total of 25–36 carcasses per species were sampled at active colonies of Adélie Pygoscelis adeliae, Gentoo P. papua, and Chinstrap P. antarcticus penguins. A linear mixed model showed that δ13C values varied significantly between tissues, presumably due to tissue-specific isotopic discrimination. In contrast, the only tissue with significantly different δ15N values was toenail. Stable isotope data revealed dietary differences among species, with Gentoo Penguins having higher average isotopic values in tissues compared to Adélie and Chinstrap penguins. In addition, Chinstrap Penguins showed a consistent, but not statistically significant, trend in having higher δ13C values compared to Adélie Penguins. Gentoo Penguins displayed the highest isotopic variability of any species for all tissues. Isotopic composition was most variable in skin in all three species making skin the least reliable tissue for isotope analysis, whereas isotopic values were least variable in toenails. Comparison of isotopic values between two bones (tibiotarsus and coracoid) showed no significant differences in isotopic values, indicating that when the same bone is not available for sampling from carcasses, sampling of any major skeletal element is likely to provide a meaningful comparison. These results allow for more informed opportunistic sampling to accurately estimate and compare penguin diet among species and between ancient and active colonies. |
format |
Dataset |
author |
Ciriani, Yanina Emslie, Steven |
author_facet |
Ciriani, Yanina Emslie, Steven |
author_sort |
Ciriani, Yanina |
title |
Stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in Antarctica |
title_short |
Stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in Antarctica |
title_full |
Stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in Antarctica |
title_fullStr |
Stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in Antarctica |
title_full_unstemmed |
Stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in Antarctica |
title_sort |
stable isotope analysis of multiple tissues from chick carcasses of three pygoscelid penguins in antarctica |
publisher |
U.S. Antarctic Program (USAP) Data Center |
publishDate |
2020 |
url |
https://dx.doi.org/10.15784/601382 https://www.usap-dc.org/view/dataset/601382 |
long_lat |
ENVELOPE(-58.000,-58.000,-62.083,-62.083) |
geographic |
Austral King George Island 25 de Mayo |
geographic_facet |
Austral King George Island 25 de Mayo |
genre |
Antarc* Antarctica antarcticus King George Island Pygoscelis adeliae |
genre_facet |
Antarc* Antarctica antarcticus King George Island Pygoscelis adeliae |
op_rights |
Creative Commons Attribution-NonCommercial-Share Alike 3.0 United States [CC BY-NC-SA 3.0] https://creativecommons.org/licenses/by-nc-sa/3.0/us/ |
op_rightsnorm |
CC-BY-NC-SA |
op_doi |
https://doi.org/10.15784/601382 |
_version_ |
1766008873314942976 |