Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia
Soil horizons below 30cm depth contain about 60% of the organic carbon stored in soils. Although insight into the physical and chemical stabilization of soil organic matter (SOM) and into microbial community composition in these horizons is being gained, information on microbial functions of subsoil...
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Amsterdam : Elsevier
2015
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Online Access: | https://dx.doi.org/10.15488/869 http://www.repo.uni-hannover.de/handle/123456789/893 |
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ftdatacite:10.15488/869 2023-05-15T17:57:46+02:00 Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia Schnecker, Jörg Wild, Birgit Takriti, Mounir Eloy Alves, Ricardo J. Gentsch, Norman Gittel, Antje Hofer, Angelika Klaus, Karoline Knoltsch, Anna Lashchinskiy, Nikolay Mikutta, Robert Richter, Andreas 2015 https://dx.doi.org/10.15488/869 http://www.repo.uni-hannover.de/handle/123456789/893 eng unknown Amsterdam : Elsevier CC BY 4.0 Unported https://creativecommons.org/licenses/by/4.0 CC-BY Amino acids Ecology Ecosystems Enzyme activity Fatty acids Forestry Microorganisms Minerals Organic carbon Permafrost Phospholipids Soil surveys Soils Stabilization Boreal forests Extracellular enzymes PLFA Steppe Tundra Enzymes boreal forest community composition enzyme activity latitudinal gradient microbial community permafrost soil depth soil horizon soil microorganism subsoil topsoil Siberia Dewey Decimal Classification500 | Naturwissenschaften540 | Chemie Dewey Decimal Classification500 | Naturwissenschaften570 | Biowissenschaften, Biologie Other CreativeWork article 2015 ftdatacite https://doi.org/10.15488/869 2021-11-05T12:55:41Z Soil horizons below 30cm depth contain about 60% of the organic carbon stored in soils. Although insight into the physical and chemical stabilization of soil organic matter (SOM) and into microbial community composition in these horizons is being gained, information on microbial functions of subsoil microbial communities and on associated microbially-mediated processes remains sparse. To identify possible controls on enzyme patterns, we correlated enzyme patterns with biotic and abiotic soil parameters, as well as with microbial community composition, estimated using phospholipid fatty acid profiles. Enzyme patterns (i.e. distance-matrixes calculated from these enzyme activities) were calculated from the activities of six extracellular enzymes (cellobiohydrolase, leucine-amino-peptidase, N-acetylglucosaminidase, chitotriosidase, phosphatase and phenoloxidase), which had been measured in soil samples from organic topsoil horizons, mineral topsoil horizons, and mineral subsoil horizons from seven ecosystems along a 1500km latitudinal transect in Western Siberia. We found that hydrolytic enzyme activities decreased rapidly with depth, whereas oxidative enzyme activities in mineral horizons were as high as, or higher than in organic topsoil horizons. Enzyme patterns varied more strongly between ecosystems in mineral subsoil horizons than in organic topsoils. The enzyme patterns in topsoil horizons were correlated with SOM content (i.e., C and N content) and microbial community composition. In contrast, the enzyme patterns in mineral subsoil horizons were related to water content, soil pH and microbial community composition. The lack of correlation between enzyme patterns and SOM quantity in the mineral subsoils suggests that SOM chemistry, spatial separation or physical stabilization of SOM rather than SOM content might determine substrate availability for enzymatic breakdown. The correlation of microbial community composition and enzyme patterns in all horizons, suggests that microbial community composition shapes enzyme patterns and might act as a modifier for the usual dependency of decomposition rates on SOM content or C/N ratios. Article in Journal/Newspaper permafrost Tundra Siberia DataCite Metadata Store (German National Library of Science and Technology) Dewey ENVELOPE(-64.320,-64.320,-65.907,-65.907) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Amino acids Ecology Ecosystems Enzyme activity Fatty acids Forestry Microorganisms Minerals Organic carbon Permafrost Phospholipids Soil surveys Soils Stabilization Boreal forests Extracellular enzymes PLFA Steppe Tundra Enzymes boreal forest community composition enzyme activity latitudinal gradient microbial community permafrost soil depth soil horizon soil microorganism subsoil topsoil Siberia Dewey Decimal Classification500 | Naturwissenschaften540 | Chemie Dewey Decimal Classification500 | Naturwissenschaften570 | Biowissenschaften, Biologie |
spellingShingle |
Amino acids Ecology Ecosystems Enzyme activity Fatty acids Forestry Microorganisms Minerals Organic carbon Permafrost Phospholipids Soil surveys Soils Stabilization Boreal forests Extracellular enzymes PLFA Steppe Tundra Enzymes boreal forest community composition enzyme activity latitudinal gradient microbial community permafrost soil depth soil horizon soil microorganism subsoil topsoil Siberia Dewey Decimal Classification500 | Naturwissenschaften540 | Chemie Dewey Decimal Classification500 | Naturwissenschaften570 | Biowissenschaften, Biologie Schnecker, Jörg Wild, Birgit Takriti, Mounir Eloy Alves, Ricardo J. Gentsch, Norman Gittel, Antje Hofer, Angelika Klaus, Karoline Knoltsch, Anna Lashchinskiy, Nikolay Mikutta, Robert Richter, Andreas Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia |
topic_facet |
Amino acids Ecology Ecosystems Enzyme activity Fatty acids Forestry Microorganisms Minerals Organic carbon Permafrost Phospholipids Soil surveys Soils Stabilization Boreal forests Extracellular enzymes PLFA Steppe Tundra Enzymes boreal forest community composition enzyme activity latitudinal gradient microbial community permafrost soil depth soil horizon soil microorganism subsoil topsoil Siberia Dewey Decimal Classification500 | Naturwissenschaften540 | Chemie Dewey Decimal Classification500 | Naturwissenschaften570 | Biowissenschaften, Biologie |
description |
Soil horizons below 30cm depth contain about 60% of the organic carbon stored in soils. Although insight into the physical and chemical stabilization of soil organic matter (SOM) and into microbial community composition in these horizons is being gained, information on microbial functions of subsoil microbial communities and on associated microbially-mediated processes remains sparse. To identify possible controls on enzyme patterns, we correlated enzyme patterns with biotic and abiotic soil parameters, as well as with microbial community composition, estimated using phospholipid fatty acid profiles. Enzyme patterns (i.e. distance-matrixes calculated from these enzyme activities) were calculated from the activities of six extracellular enzymes (cellobiohydrolase, leucine-amino-peptidase, N-acetylglucosaminidase, chitotriosidase, phosphatase and phenoloxidase), which had been measured in soil samples from organic topsoil horizons, mineral topsoil horizons, and mineral subsoil horizons from seven ecosystems along a 1500km latitudinal transect in Western Siberia. We found that hydrolytic enzyme activities decreased rapidly with depth, whereas oxidative enzyme activities in mineral horizons were as high as, or higher than in organic topsoil horizons. Enzyme patterns varied more strongly between ecosystems in mineral subsoil horizons than in organic topsoils. The enzyme patterns in topsoil horizons were correlated with SOM content (i.e., C and N content) and microbial community composition. In contrast, the enzyme patterns in mineral subsoil horizons were related to water content, soil pH and microbial community composition. The lack of correlation between enzyme patterns and SOM quantity in the mineral subsoils suggests that SOM chemistry, spatial separation or physical stabilization of SOM rather than SOM content might determine substrate availability for enzymatic breakdown. The correlation of microbial community composition and enzyme patterns in all horizons, suggests that microbial community composition shapes enzyme patterns and might act as a modifier for the usual dependency of decomposition rates on SOM content or C/N ratios. |
format |
Article in Journal/Newspaper |
author |
Schnecker, Jörg Wild, Birgit Takriti, Mounir Eloy Alves, Ricardo J. Gentsch, Norman Gittel, Antje Hofer, Angelika Klaus, Karoline Knoltsch, Anna Lashchinskiy, Nikolay Mikutta, Robert Richter, Andreas |
author_facet |
Schnecker, Jörg Wild, Birgit Takriti, Mounir Eloy Alves, Ricardo J. Gentsch, Norman Gittel, Antje Hofer, Angelika Klaus, Karoline Knoltsch, Anna Lashchinskiy, Nikolay Mikutta, Robert Richter, Andreas |
author_sort |
Schnecker, Jörg |
title |
Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia |
title_short |
Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia |
title_full |
Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia |
title_fullStr |
Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia |
title_full_unstemmed |
Microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in Western Siberia |
title_sort |
microbial community composition shapes enzyme patterns in topsoil and subsoil horizons along a latitudinal transect in western siberia |
publisher |
Amsterdam : Elsevier |
publishDate |
2015 |
url |
https://dx.doi.org/10.15488/869 http://www.repo.uni-hannover.de/handle/123456789/893 |
long_lat |
ENVELOPE(-64.320,-64.320,-65.907,-65.907) |
geographic |
Dewey |
geographic_facet |
Dewey |
genre |
permafrost Tundra Siberia |
genre_facet |
permafrost Tundra Siberia |
op_rights |
CC BY 4.0 Unported https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.15488/869 |
_version_ |
1766166261260091392 |