Stimulation of adventitious root formation by laser wounding in rose cuttings: A matter of energy and pattern ...

• Main Authors: Morales-Orellana, Raul Javier, Winkelmann, Traud, Bettin, Andreas, Rath, Thomas
• Format: Text
• Language: English
• Published: Lausanne : Frontiers Media 2022
• Subjects: adventitious root formation; histology; laser ablation; Rosa canina; stem cutting; wounding; Dewey Decimal Classification500 | Naturwissenschaften570 | Biowissenschaften, Biologie; Dewey; Beluga; Beluga*
• Online Access: https://dx.doi.org/10.15488/14011; https://www.repo.uni-hannover.de/handle/123456789/14125

Adventitious root (AR) formation is the basis of vegetative propagation in rose, be it via stem cuttings or via stenting. During this process, wounding plays a pivotal role since cell reprogramming takes place at the tissue adjacent to the wound. We investigated the effects of wounding on AR formation on leafy single-node stem cuttings of the rose rootstock R. canina ‘Pfänder’ (codes R02-3 and R02-6) and the cut rose cultivar Rosa ‘Tan09283’ (Registration name ‘Beluga’). Laser wounding treatments were based on the assisted removal of tissue layers located in the bark. The positioning of wounding was studied based on two marking directions: along the cutting base (strip pattern) and around the cutting base (ring pattern). Additionally, the effects of external supply of indole-butyric acid (IBA 1 mg L-1) on rooting were analyzed. Results showed that in order to remove specific tissue layers, the calculation of the laser energy density (J cm-2) in terms of cutting diameter was necessary. Interestingly, the ...