Complete genome sequence of Thermus brockianus GE-1 reveals key enzymes of xylan/xylose metabolism ...

• Main Authors: Schäfers, Christian, Blank, Saskia, Wiebusch, Sigrid, Elleuche, Skander, Antranikian, Garabed
• Format: Article in Journal/Newspaper
• Language: English
• Published: BioMed Central 2017
• Subjects: whole genome sequence; de novo assembly; Thermus; Thermus brockianus; xylan degradation; xylose metabolism; Thermophiles; single molecule real-time sequencing; Biowissenschaften, Biologie; Geysir; Iceland
• Online Access: https://dx.doi.org/10.15480/882.1407; https://tore.tuhh.de/handle/11420/1410

Thermus brockianus strain GE-1 is a thermophilic, Gram-negative, rod-shaped and non-motile bacterium that was isolated from the Geysir geothermal area, Iceland. Like other thermophiles, Thermus species are often used as model organisms to understand the mechanism of action of extremozymes, especially focusing on their heat-activity and thermostability. Genome-specific features of T. brockianus GE-1 and their properties further help to explain processes of the adaption of extremophiles at elevated temperatures. Here we analyze the first whole genome sequence of T. brockianus strain GE-1. Insights of the genome sequence and the methodologies that were applied during de novo assembly and annotation are given in detail. The finished genome shows a phred quality value of QV50. The complete genome size is 2.38 Mb, comprising the chromosome (2,035,182 bp), the megaplasmid pTB1 (342,792 bp) and the smaller plasmid pTB2 (10,299 bp). Gene prediction revealed 2,511 genes in total, including 2,458 protein-encoding ...