Preservation of ancient eukaryotic DNA in methane hydrate-associated marine sediments. ...

• Main Author: Kyoto University, Graduate School Of Human
• Format: Dataset
• Language: English
• Published: MGnify 2020
• Subjects: Methane hydrate
• Online Access: https://dx.doi.org/10.15468/vsczdo; https://www.gbif.org/dataset/2eafa2ef-7b0b-46e8-bae9-9907dacefcf5

Ancient eukaryotic DNA in marine sediment can provide valuable information on the paleo-environment. However, labile nucleic acids are generally considered to be microbially degraded in water and sediment columns. Previously, ancient eukaryotic DNA has been retrieved from sediments associated with anoxic water column in lake and semi-closed sea, due to the minimized effect of oxic biodegradation. Cold seep sediment is characterized by high upward flux of methane, where there is the possibility that the excess energy source might suppress the degradation of deposited organic matter including nucleic acids by microbial activity. We investigated the preservation of ancient eukaryotic DNA in marine sediments associated with and without methane hydrate in the eastern Japan Sea, between which the dominant prokaryotic populations are clearly distinct. Marine sediments were retrieved from the eastern Japan Sea during a cruise onboard R/V Marion Dufresne in June 2010. Two-step alkaline DNA extractions were conducted ...