Cryoconite sediments Targeted Locus (Loci) ...
Biological processes on glacier surfaces affect the physical behaviour of glaciers and ice sheets and carbon and nutrient fluxes in the cryosphere. Changes in rates of biological processes are often associated with changes in diversity, yet there is little knowledge of the diversity of active microb...
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Online Access: | https://dx.doi.org/10.15468/qmjm6v https://www.gbif.org/dataset/57bdb985-4a64-4b8d-99f0-c000d77d1b90 |
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ftdatacite:10.15468/qmjm6v 2023-12-31T10:07:08+01:00 Cryoconite sediments Targeted Locus (Loci) ... Thomasgbif 2019 https://dx.doi.org/10.15468/qmjm6v https://www.gbif.org/dataset/57bdb985-4a64-4b8d-99f0-c000d77d1b90 en eng MGnify Creative Commons Attribution (CC-BY) 4.0 http://creativecommons.org/licenses/by/4.0/legalcode Metagenomics environmental genomics Dataset SAMPLING_EVENT dataset 2019 ftdatacite https://doi.org/10.15468/qmjm6v 2023-12-01T10:29:57Z Biological processes on glacier surfaces affect the physical behaviour of glaciers and ice sheets and carbon and nutrient fluxes in the cryosphere. Changes in rates of biological processes are often associated with changes in diversity, yet there is little knowledge of the diversity of active microbes on glacier surfaces and its controls. We examined the microbial abundance, community structure, and the proportion of the active microbes at two contrasting surface sites located at the margin and in the interior of the Greenland ice sheet over the course of a melting season, using DNA and RNA co-extraction from the same samples, quantitative PCR and pyrosequencing of 16S rRNA and rDNA. Whereas the rDNA abundances were similar at both sites (108 109 copies g-1), the rRNA abundance was significantly higher in the interior (1010 copies g-1). Significant differences in diversity were found between the sites, with the DNA diversity significantly higher at the margin and the RNA diversity higher at the interior ... Dataset glacier Greenland Ice Sheet DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
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DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Metagenomics environmental genomics |
spellingShingle |
Metagenomics environmental genomics Thomasgbif Cryoconite sediments Targeted Locus (Loci) ... |
topic_facet |
Metagenomics environmental genomics |
description |
Biological processes on glacier surfaces affect the physical behaviour of glaciers and ice sheets and carbon and nutrient fluxes in the cryosphere. Changes in rates of biological processes are often associated with changes in diversity, yet there is little knowledge of the diversity of active microbes on glacier surfaces and its controls. We examined the microbial abundance, community structure, and the proportion of the active microbes at two contrasting surface sites located at the margin and in the interior of the Greenland ice sheet over the course of a melting season, using DNA and RNA co-extraction from the same samples, quantitative PCR and pyrosequencing of 16S rRNA and rDNA. Whereas the rDNA abundances were similar at both sites (108 109 copies g-1), the rRNA abundance was significantly higher in the interior (1010 copies g-1). Significant differences in diversity were found between the sites, with the DNA diversity significantly higher at the margin and the RNA diversity higher at the interior ... |
format |
Dataset |
author |
Thomasgbif |
author_facet |
Thomasgbif |
author_sort |
Thomasgbif |
title |
Cryoconite sediments Targeted Locus (Loci) ... |
title_short |
Cryoconite sediments Targeted Locus (Loci) ... |
title_full |
Cryoconite sediments Targeted Locus (Loci) ... |
title_fullStr |
Cryoconite sediments Targeted Locus (Loci) ... |
title_full_unstemmed |
Cryoconite sediments Targeted Locus (Loci) ... |
title_sort |
cryoconite sediments targeted locus (loci) ... |
publisher |
MGnify |
publishDate |
2019 |
url |
https://dx.doi.org/10.15468/qmjm6v https://www.gbif.org/dataset/57bdb985-4a64-4b8d-99f0-c000d77d1b90 |
genre |
glacier Greenland Ice Sheet |
genre_facet |
glacier Greenland Ice Sheet |
op_rights |
Creative Commons Attribution (CC-BY) 4.0 http://creativecommons.org/licenses/by/4.0/legalcode |
op_doi |
https://doi.org/10.15468/qmjm6v |
_version_ |
1786839367876083712 |