Pyrosequencing reveals effect of increased pCO2 on bacterial assemblage shifts in response to glucose addition in Fram Strait seawater mesocosms ...

• Main Author: UIB-UNIRES-MILJO
• Format: Dataset
• Language: English
• Published: MGnify 2022
• Subjects: East Greenland; east greenland current; Fram Strait; Greenland; Ocean acidification; Shannon
• Online Access: https://dx.doi.org/10.15468/fkxsas; https://www.gbif.org/dataset/1cc7ea6d-ae95-482d-932d-3d414978ff14

Ocean acidification (OA) may stimulate primary production through increased availability of inorganic carbon in the photic zone, which may in turn change the biogenic flux of dissolved organic carbon (DOC) and the growth potential of heterotrophic bacteria. In order to investigate the effects of OA on marine bacterial assemblages, a two-by-three factorial mescosom experiment was conducted using surface seawater from the East Greenland Current in Fram Strait. Pyrosequencing of the V1-V2 region of bacterial 16S ribosomal RNA genes was used to investigate differences in the endpoint (Day 9) composition of bacterial assemblages in mineral nutrient-replete mesocosms amended with glucose (0 M, 5.3 M and 15.9 M) under ambient (250 atm) or acidified (400 atm) partial pressure of CO2 (pCO2). All mesocosms showed low richness and evenness by Chao1-estimator and Shannon-Wiener diversity index, respectively, with general dominance by Gammaproteobacteria and Flavobacteria. Non-metric multidimensional scaling analysis and ...