Expression, purification and characterization of neuraminidase from avian flu virus (H5N1) in Pichia pastoris ...
The avian influenza A (H5N1) virus is highly contagious in both wild bird populations and domestic poultry flocks in various continents posing a serious threat to human health. Previous studies revealed that the major surface glycoprotein of the virion namely, neuraminidase (NA) plays an important r...
Main Author: | |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
Chulalongkorn University
2011
|
Subjects: | |
Online Access: | https://dx.doi.org/10.14457/cu.the.2011.1718 http://doi.nrct.go.th/?page=resolve_doi&resolve_doi=10.14457/CU.the.2011.1718 |
id |
ftdatacite:10.14457/cu.the.2011.1718 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.14457/cu.the.2011.1718 2023-08-27T04:08:34+02:00 Expression, purification and characterization of neuraminidase from avian flu virus (H5N1) in Pichia pastoris ... Puchita Rerksongkrou 2011 https://dx.doi.org/10.14457/cu.the.2011.1718 http://doi.nrct.go.th/?page=resolve_doi&resolve_doi=10.14457/CU.the.2011.1718 unknown Chulalongkorn University Avian influenza Neuraminidase dataset Dataset 2011 ftdatacite https://doi.org/10.14457/cu.the.2011.1718 2023-08-07T14:24:23Z The avian influenza A (H5N1) virus is highly contagious in both wild bird populations and domestic poultry flocks in various continents posing a serious threat to human health. Previous studies revealed that the major surface glycoprotein of the virion namely, neuraminidase (NA) plays an important role in viral replication and infection. The NA is a mushroom-shaped spike protein composed of a single polypeptide chain with three distinct domains: head domain containing the enzyme active site, a highly variable stalk region, and N- terminal signal anchor domain by which the enzyme is embedded in the viral envelope. The function is to hydrolyze the terminal sialic acids of sialoglycans and promotes the release of progeny virus from an infected host cell by destroying receptors on the host cell and the virus itself. The inhibition of NA activity will limit the spread of viral infection thereby suppressing the onset of disease. In this research, the full length and globular head domain of NA were expressed in ... Dataset Avian flu DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
unknown |
topic |
Avian influenza Neuraminidase |
spellingShingle |
Avian influenza Neuraminidase Puchita Rerksongkrou Expression, purification and characterization of neuraminidase from avian flu virus (H5N1) in Pichia pastoris ... |
topic_facet |
Avian influenza Neuraminidase |
description |
The avian influenza A (H5N1) virus is highly contagious in both wild bird populations and domestic poultry flocks in various continents posing a serious threat to human health. Previous studies revealed that the major surface glycoprotein of the virion namely, neuraminidase (NA) plays an important role in viral replication and infection. The NA is a mushroom-shaped spike protein composed of a single polypeptide chain with three distinct domains: head domain containing the enzyme active site, a highly variable stalk region, and N- terminal signal anchor domain by which the enzyme is embedded in the viral envelope. The function is to hydrolyze the terminal sialic acids of sialoglycans and promotes the release of progeny virus from an infected host cell by destroying receptors on the host cell and the virus itself. The inhibition of NA activity will limit the spread of viral infection thereby suppressing the onset of disease. In this research, the full length and globular head domain of NA were expressed in ... |
format |
Dataset |
author |
Puchita Rerksongkrou |
author_facet |
Puchita Rerksongkrou |
author_sort |
Puchita Rerksongkrou |
title |
Expression, purification and characterization of neuraminidase from avian flu virus (H5N1) in Pichia pastoris ... |
title_short |
Expression, purification and characterization of neuraminidase from avian flu virus (H5N1) in Pichia pastoris ... |
title_full |
Expression, purification and characterization of neuraminidase from avian flu virus (H5N1) in Pichia pastoris ... |
title_fullStr |
Expression, purification and characterization of neuraminidase from avian flu virus (H5N1) in Pichia pastoris ... |
title_full_unstemmed |
Expression, purification and characterization of neuraminidase from avian flu virus (H5N1) in Pichia pastoris ... |
title_sort |
expression, purification and characterization of neuraminidase from avian flu virus (h5n1) in pichia pastoris ... |
publisher |
Chulalongkorn University |
publishDate |
2011 |
url |
https://dx.doi.org/10.14457/cu.the.2011.1718 http://doi.nrct.go.th/?page=resolve_doi&resolve_doi=10.14457/CU.the.2011.1718 |
genre |
Avian flu |
genre_facet |
Avian flu |
op_doi |
https://doi.org/10.14457/cu.the.2011.1718 |
_version_ |
1775349400466358272 |