Raw DNA sequences from a high-Arctic time series station in Isfjorden ...

• Main Authors: Chitkara, Cheshtaa, Gabrielsen, Tove M., Marquardt, Miriam, Vader, Anna, Søreide, Janne
• Format: Dataset
• Language: unknown
• Published: Archive2014 2024
• Subjects: Natural sciences; FOS: Natural sciences; Biology; Marine Biology; Adventfjorden; Arctic; Svalbard; Isfjord*; Isfjorden; UNIS; University Centre in Svalbard
• Online Access: https://dx.doi.org/10.11582/2024.00129; https://archive.sigma2.no/pages/public/datasetDetail.jsf?id=10.11582/2024.00129

Time series of Raw DNA metabarcoding sequences from the Isfjorden Adventfjorden (IsA) marine time series, run by the University Centre in Svalbard (UNIS) from December 2011 until present. The IsA time series (https://www.unis.no/project/isa/) is a model system to study seasonal dynamics, interannual variability and long term changes in a high-Arctic marine ecosystem impacted by Atlantic water inflow. Samples have been taken once every week in 2012, once every two weeks in 2013 and once every month since 2014 until 2019. Sea water samples collected with the help of niskin bottles were first pre-filtered using a 10 μm mesh, and further filtered using a 0.45 μm filter. Organisms in the size range 10-0.45 μm were further studied. DNA was extracted using the DNeasy Plant Mini Kit, using the manufacturer´s protocol with some modifications. DNA extracts with concentration above 1 ng/µl were sent for high-throughput 300 bp paired-end Illumina MiSeq sequencing to amplify the V4 region of the nuclear 18s rRNA gene ... : 6 null, totaling ...... 18.56 KB 1 gzip compressed data, max speed, from NTFS filesystem (NT), original size 48293, totaling ...... 514.3 KB 1 gzip compressed data, max speed, from NTFS filesystem (NT), original size 14548, totaling ...... 338.82 KB 1 gzip compressed data, max speed, from NTFS filesystem (NT), original size 21160, totaling ...... 426.48 KB 1 gzip compressed data, max speed, from NTFS filesystem (NT), original size 19171, totaling ...... 290.16 KB 1 gzip compressed data, max speed, from NTFS filesystem (NT), original size 48243, totaling ...... 583.54 KB 16 ASCII text, with no line terminators, totaling ...... 512 B 1 gzip compressed data, max speed, from NTFS filesystem (NT), original size 28426, totaling ...... 306.7 KB 1 gzip compressed data, max speed, from NTFS filesystem (NT), original size 28448, totaling ...... 263 KB 1 ASCII text, totaling ...... 14.28 KB 1 gzip compressed data, max speed, from NTFS filesystem (NT), original size 19181, totaling ...... 246.33 KB 1 gzip compressed ...