18S metabarcoding of Arctic meiobenthic nematodes ...

• Main Authors: Flo, Snorre, Svalbard, The University Centre In
• Format: Dataset
• Language: unknown
• Published: Archive2014 2024
• Subjects: Natural sciences; FOS Natural sciences; Biology; Marine Biology; Barents Sea; Nansen Basin
• Online Access: https://dx.doi.org/10.11582/2024.00105; https://archive.sigma2.no/pages/public/datasetDetail.jsf?id=10.11582/2024.00105

The nema_novaseq_raw dataset contains DNA reads from a total of 156 samples including extraction negatives, and nematodes. The purpose of the study was to investigate the trophic interactions of Arctic meiobenthic nematodes using a "brute force" prey metabarcoding approach (Flo et al., 2024). Sediment was collected on four locations (process stations P1, P4, P6 and P7) along a transect from the Barents Sea to the Arctic Nansen Basin in August 2019 (Q3), December 2019 (Q4), March 2021 (Q1) and April/May 2021 (Q2) with a box-corer, and fixed on ice-cold ethanol (96%, -20 deg C). Up to 11 biological replicates of nematodes were picked from each station from each cruise, and the samples are named accordingly (i.e. nm_1_7_11 contains a nematode from cruise Q1, station P7, and biological replicate number 11). Replicate number 12 in each set if samples (i.e. nm_1_7_12) are always extraction negatives. DNA from picked specimen were extracted using the E.Z.N.A Tissue DNA kit (Omega Bio-Tek), and tested for ... : 5 data, totaling ...... 436.68 GB ...