Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou ...
Chronic wasting disease (CWD) is a transmissible prion disease affecting cervids mainly in North America. The responsible agent for this disease is the protease-resistant and infectious prion protein isoform (PrPSc) which is converted from a normal host-encoded glycoprotein (PrPC). In this study, I...
Main Author: | |
---|---|
Format: | Master Thesis |
Language: | English |
Published: |
Graduate Studies
2016
|
Subjects: | |
Online Access: | https://dx.doi.org/10.11575/prism/25326 https://prism.ucalgary.ca/handle/11023/2896 |
id |
ftdatacite:10.11575/prism/25326 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.11575/prism/25326 2023-11-05T03:41:14+01:00 Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou ... Cheng, Yo Ching 2016 https://dx.doi.org/10.11575/prism/25326 https://prism.ucalgary.ca/handle/11023/2896 en eng Graduate Studies University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. Animal Pathology Genetics FOS Biological sciences Biology--Molecular Veterinary Science Chronic Wasting Disease Prions Prion diseases diagnosis Caribou Elk Surveillance RT-QuIC article master thesis CreativeWork Other 2016 ftdatacite https://doi.org/10.11575/prism/25326 2023-10-09T10:53:16Z Chronic wasting disease (CWD) is a transmissible prion disease affecting cervids mainly in North America. The responsible agent for this disease is the protease-resistant and infectious prion protein isoform (PrPSc) which is converted from a normal host-encoded glycoprotein (PrPC). In this study, I employed a novel in vitro amplification assay; the real-time quaking-induced conversion assay (RT-QuIC) to detect CWD prions in feces. I demonstrated the use of optimized RT-QuIC to define the shedding pattern of CWD prions in feces during the course of CWD. Additionally, I analyzed the distribution of PrP polymorphism among caribou populations in Alberta. The results permit predictions on the probability of CWD transmission to caribou populations. My studies provide a new tool to improve CWD surveillance and transmission risk assessment, and may be useful for CWD management in the future. ... Master Thesis caribou DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Animal Pathology Genetics FOS Biological sciences Biology--Molecular Veterinary Science Chronic Wasting Disease Prions Prion diseases diagnosis Caribou Elk Surveillance RT-QuIC |
spellingShingle |
Animal Pathology Genetics FOS Biological sciences Biology--Molecular Veterinary Science Chronic Wasting Disease Prions Prion diseases diagnosis Caribou Elk Surveillance RT-QuIC Cheng, Yo Ching Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou ... |
topic_facet |
Animal Pathology Genetics FOS Biological sciences Biology--Molecular Veterinary Science Chronic Wasting Disease Prions Prion diseases diagnosis Caribou Elk Surveillance RT-QuIC |
description |
Chronic wasting disease (CWD) is a transmissible prion disease affecting cervids mainly in North America. The responsible agent for this disease is the protease-resistant and infectious prion protein isoform (PrPSc) which is converted from a normal host-encoded glycoprotein (PrPC). In this study, I employed a novel in vitro amplification assay; the real-time quaking-induced conversion assay (RT-QuIC) to detect CWD prions in feces. I demonstrated the use of optimized RT-QuIC to define the shedding pattern of CWD prions in feces during the course of CWD. Additionally, I analyzed the distribution of PrP polymorphism among caribou populations in Alberta. The results permit predictions on the probability of CWD transmission to caribou populations. My studies provide a new tool to improve CWD surveillance and transmission risk assessment, and may be useful for CWD management in the future. ... |
format |
Master Thesis |
author |
Cheng, Yo Ching |
author_facet |
Cheng, Yo Ching |
author_sort |
Cheng, Yo Ching |
title |
Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou ... |
title_short |
Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou ... |
title_full |
Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou ... |
title_fullStr |
Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou ... |
title_full_unstemmed |
Chronic Wasting Disease: Investigations on Prion Shedding and Risk of Transmission to Caribou ... |
title_sort |
chronic wasting disease: investigations on prion shedding and risk of transmission to caribou ... |
publisher |
Graduate Studies |
publishDate |
2016 |
url |
https://dx.doi.org/10.11575/prism/25326 https://prism.ucalgary.ca/handle/11023/2896 |
genre |
caribou |
genre_facet |
caribou |
op_rights |
University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. |
op_doi |
https://doi.org/10.11575/prism/25326 |
_version_ |
1781697566727995392 |