Data from: Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1
Panagrolaimus sp. DAW1 is a freeze-tolerant Antarctic nematode which survives extensive intracellular ice formation. The molecular mechanisms of this extreme adaptation are still poorly understood. We recently showed that desiccation-enhanced RNA interference (RNAi) soaking can be used in conjunctio...
Main Authors: | , , , |
---|---|
Language: | unknown |
Published: |
2017
|
Subjects: | |
Online Access: | http://nbn-resolving.org/urn:nbn:nl:ui:13-vx-r8ox https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99865 |
id |
ftdans:oai:easy.dans.knaw.nl:easy-dataset:99865 |
---|---|
record_format |
openpolar |
spelling |
ftdans:oai:easy.dans.knaw.nl:easy-dataset:99865 2023-07-02T03:30:18+02:00 Data from: Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 Seybold, A.C. Wharton, David A. Thorne, Michael A.S. Marshall, Craig J. 2017-11-29T18:13:42.000+01:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-vx-r8ox https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99865 unknown doi:10.5061/dryad.bc276/1 doi:10.1242/bio.023341 http://nbn-resolving.org/urn:nbn:nl:ui:13-vx-r8ox doi:10.5061/dryad.bc276 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99865 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2017 ftdans https://doi.org/10.5061/dryad.bc276/110.1242/bio.02334110.5061/dryad.bc276 2023-06-13T13:25:59Z Panagrolaimus sp. DAW1 is a freeze-tolerant Antarctic nematode which survives extensive intracellular ice formation. The molecular mechanisms of this extreme adaptation are still poorly understood. We recently showed that desiccation-enhanced RNA interference (RNAi) soaking can be used in conjunction with quantitative polymerase chain reaction (qPCR) to screen for phenotypes associated with reduced expression of candidate genes in Panagrolaimus sp. DAW1. Here, we present the use of this approach to investigate the role of trehalose synthesis genes in this remarkable organism. Previous studies have shown that acclimating Panagrolaimus sp. DAW1 at 5°C before freezing or desiccation substantially enhances survival. In this study, the expression of tps-2 and other genes associated with trehalose metabolism, as well as lea-1, hsp-70 and gpx-1, in cold-acclimated and non-acclimated nematodes was analyzed using qPCR. Pd-tps-2 and Pd-lea-1 were significantly upregulated after cold acclimation, indicating an inducible expression in the cold adaptation of Panagrolaimus sp. DAW1. The role of trehalose synthesis genes in Panagrolaimus sp. DAW1 was further investigated by RNAi. Compared to the controls, Pd-tps-2a(RNAi)-treated and cold-acclimated nematodes showed a significant decrease in mRNA, but no change in trehalose content or freezing survival. The involvement of two other trehalose synthesis genes (tps-2b and gob-1) was also investigated. These findings provide the first functional genomic investigation of trehalose synthesis genes in the non-model organism Panagrolaimus sp. DAW1. The presence of several trehalose synthesis genes with different RNAi sensitivities suggests the existence of multiple backup systems in Panagrolaimus sp. DAW1, underlining the importance of this sugar in preparation for freezing. Other/Unknown Material Antarc* Antarctic Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Antarctic The Antarctic |
institution |
Open Polar |
collection |
Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Seybold, A.C. Wharton, David A. Thorne, Michael A.S. Marshall, Craig J. Data from: Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 |
topic_facet |
Life sciences medicine and health care |
description |
Panagrolaimus sp. DAW1 is a freeze-tolerant Antarctic nematode which survives extensive intracellular ice formation. The molecular mechanisms of this extreme adaptation are still poorly understood. We recently showed that desiccation-enhanced RNA interference (RNAi) soaking can be used in conjunction with quantitative polymerase chain reaction (qPCR) to screen for phenotypes associated with reduced expression of candidate genes in Panagrolaimus sp. DAW1. Here, we present the use of this approach to investigate the role of trehalose synthesis genes in this remarkable organism. Previous studies have shown that acclimating Panagrolaimus sp. DAW1 at 5°C before freezing or desiccation substantially enhances survival. In this study, the expression of tps-2 and other genes associated with trehalose metabolism, as well as lea-1, hsp-70 and gpx-1, in cold-acclimated and non-acclimated nematodes was analyzed using qPCR. Pd-tps-2 and Pd-lea-1 were significantly upregulated after cold acclimation, indicating an inducible expression in the cold adaptation of Panagrolaimus sp. DAW1. The role of trehalose synthesis genes in Panagrolaimus sp. DAW1 was further investigated by RNAi. Compared to the controls, Pd-tps-2a(RNAi)-treated and cold-acclimated nematodes showed a significant decrease in mRNA, but no change in trehalose content or freezing survival. The involvement of two other trehalose synthesis genes (tps-2b and gob-1) was also investigated. These findings provide the first functional genomic investigation of trehalose synthesis genes in the non-model organism Panagrolaimus sp. DAW1. The presence of several trehalose synthesis genes with different RNAi sensitivities suggests the existence of multiple backup systems in Panagrolaimus sp. DAW1, underlining the importance of this sugar in preparation for freezing. |
author |
Seybold, A.C. Wharton, David A. Thorne, Michael A.S. Marshall, Craig J. |
author_facet |
Seybold, A.C. Wharton, David A. Thorne, Michael A.S. Marshall, Craig J. |
author_sort |
Seybold, A.C. |
title |
Data from: Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 |
title_short |
Data from: Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 |
title_full |
Data from: Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 |
title_fullStr |
Data from: Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 |
title_full_unstemmed |
Data from: Investigating trehalose synthesis genes after cold acclimation in the Antarctic nematode Panagrolaimus sp. DAW1 |
title_sort |
data from: investigating trehalose synthesis genes after cold acclimation in the antarctic nematode panagrolaimus sp. daw1 |
publishDate |
2017 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-vx-r8ox https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99865 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
doi:10.5061/dryad.bc276/1 doi:10.1242/bio.023341 http://nbn-resolving.org/urn:nbn:nl:ui:13-vx-r8ox doi:10.5061/dryad.bc276 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99865 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.bc276/110.1242/bio.02334110.5061/dryad.bc276 |
_version_ |
1770274511534424064 |