Data from: Identification of genomic regions associated with sex in Pacific halibut
Understanding and identifying the genetic mechanisms responsible for sex-determination are important for species management, particularly in exploited fishes where sex biased harvest could have implications on population dynamics and long-term persistence. The Pacific halibut (Hippoglossus stenolepi...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:99675 2023-07-02T03:33:40+02:00 Data from: Identification of genomic regions associated with sex in Pacific halibut Drinan, Daniel P. Loher, Timothy Hauser, Lorenz 2017-11-06T16:10:06.000+01:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-xb-d3re https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99675 unknown doi:10.5061/dryad.ff072/1 doi:10.5061/dryad.ff072/2 doi:10.5061/dryad.ff072/3 doi:10.5061/dryad.ff072/4 doi:10.1093/jhered/esx102 http://nbn-resolving.org/urn:nbn:nl:ui:13-xb-d3re doi:10.5061/dryad.ff072 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99675 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2017 ftdans https://doi.org/10.5061/dryad.ff072/110.5061/dryad.ff072/210.5061/dryad.ff072/310.5061/dryad.ff072/410.1093/jhered/esx10210.5061/dryad.ff072 2023-06-13T12:46:57Z Understanding and identifying the genetic mechanisms responsible for sex-determination are important for species management, particularly in exploited fishes where sex biased harvest could have implications on population dynamics and long-term persistence. The Pacific halibut (Hippoglossus stenolepis) supports important fisheries in the North Pacific Ocean. The proportion of each sex in the annual harvest is currently estimated using growth curves, but genetic techniques may provide a more accurate method. We used restriction-site associated DNA (RAD) sequencing to identify RAD-tags that were linked to genetic sex, based on differentiation (FST) between the sexes. Identified RAD-tags were aligned to the Atlantic halibut (Hippoglossus hippoglossus) linkage map, the turbot (Scophthalmus maximus) genome, and the half-smooth tongue sole (Cynoglossus semilaevis) genome to identify genomic regions that may be involved in sex determination. In total, 56 RAD-tags (70 single nucleotide polymorphisms) were linked to sex, and three RAD-tags were identified in only females. Sex-linked loci aligned to three linkage groups in the Atlantic halibut (LG07: 7 loci, LG15: 1 locus, and LG24: 1 locus), three chromosomes in the turbot (LG12: 13 loci, LG01: 1 locus, and LG05: 1 locus), and one chromosome in the half-smooth tongue sole (ChrZ: 9 loci). Results add support to the hypothesis that Pacific halibut genetic sex is determined in a ZW system. Two sex-linked loci were further developed into sex identification assays, and their efficacy was tested on individuals that had been morphologically sexed. The accuracy of each assay on its own was 97.5% compared to morphological sex. Other/Unknown Material Scophthalmus maximus Turbot Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Pacific |
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Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
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Life sciences medicine and health care |
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Life sciences medicine and health care Drinan, Daniel P. Loher, Timothy Hauser, Lorenz Data from: Identification of genomic regions associated with sex in Pacific halibut |
topic_facet |
Life sciences medicine and health care |
description |
Understanding and identifying the genetic mechanisms responsible for sex-determination are important for species management, particularly in exploited fishes where sex biased harvest could have implications on population dynamics and long-term persistence. The Pacific halibut (Hippoglossus stenolepis) supports important fisheries in the North Pacific Ocean. The proportion of each sex in the annual harvest is currently estimated using growth curves, but genetic techniques may provide a more accurate method. We used restriction-site associated DNA (RAD) sequencing to identify RAD-tags that were linked to genetic sex, based on differentiation (FST) between the sexes. Identified RAD-tags were aligned to the Atlantic halibut (Hippoglossus hippoglossus) linkage map, the turbot (Scophthalmus maximus) genome, and the half-smooth tongue sole (Cynoglossus semilaevis) genome to identify genomic regions that may be involved in sex determination. In total, 56 RAD-tags (70 single nucleotide polymorphisms) were linked to sex, and three RAD-tags were identified in only females. Sex-linked loci aligned to three linkage groups in the Atlantic halibut (LG07: 7 loci, LG15: 1 locus, and LG24: 1 locus), three chromosomes in the turbot (LG12: 13 loci, LG01: 1 locus, and LG05: 1 locus), and one chromosome in the half-smooth tongue sole (ChrZ: 9 loci). Results add support to the hypothesis that Pacific halibut genetic sex is determined in a ZW system. Two sex-linked loci were further developed into sex identification assays, and their efficacy was tested on individuals that had been morphologically sexed. The accuracy of each assay on its own was 97.5% compared to morphological sex. |
author |
Drinan, Daniel P. Loher, Timothy Hauser, Lorenz |
author_facet |
Drinan, Daniel P. Loher, Timothy Hauser, Lorenz |
author_sort |
Drinan, Daniel P. |
title |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_short |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_full |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_fullStr |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_full_unstemmed |
Data from: Identification of genomic regions associated with sex in Pacific halibut |
title_sort |
data from: identification of genomic regions associated with sex in pacific halibut |
publishDate |
2017 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-xb-d3re https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99675 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
doi:10.5061/dryad.ff072/1 doi:10.5061/dryad.ff072/2 doi:10.5061/dryad.ff072/3 doi:10.5061/dryad.ff072/4 doi:10.1093/jhered/esx102 http://nbn-resolving.org/urn:nbn:nl:ui:13-xb-d3re doi:10.5061/dryad.ff072 https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:99675 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.ff072/110.5061/dryad.ff072/210.5061/dryad.ff072/310.5061/dryad.ff072/410.1093/jhered/esx10210.5061/dryad.ff072 |
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1770273712316088320 |