Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments
Sea ice is an analog environment for several of astrobiology’s near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to l...
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ftdans:oai:easy.dans.knaw.nl:easy-dataset:92181 2023-07-02T03:32:27+02:00 Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments Lindensmith, Christian A. Rider, Stephanie Bedrossian, Manuel Wallace, J. Kent Serabyn, Eugene Showalter, Gordon M. Deming, Jody W. Nadeau, Jay L. 2016-02-01T22:33:49.000+01:00 http://nbn-resolving.org/urn:nbn:nl:ui:13-21-c6ja https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:92181 unknown doi:10.5061/dryad.rc63v/1 doi:10.5061/dryad.rc63v/2 doi:10.5061/dryad.rc63v/3 doi:10.5061/dryad.rc63v/4 doi:10.5061/dryad.rc63v/5 doi:10.5061/dryad.rc63v/6 doi:10.5061/dryad.rc63v/7 doi:10.5061/dryad.rc63v/8 doi:10.5061/dryad.rc63v/9 doi:10.5061/dryad.rc63v/10 doi:10.5061/dryad.rc63v/11 doi:10.1371/journal.pone.0147700 PMID:26812683 http://nbn-resolving.org/urn:nbn:nl:ui:13-21-c6ja doi:10.5061/dryad.rc63v https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:92181 OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf Life sciences medicine and health care 2016 ftdans https://doi.org/10.5061/dryad.rc63v/110.5061/dryad.rc63v/210.5061/dryad.rc63v/310.5061/dryad.rc63v/410.5061/dryad.rc63v/510.5061/dryad.rc63v/610.5061/dryad.rc63v/710.5061/dryad.rc63v/810.5061/dryad.rc63v/910.5061/dryad.rc63v/1010.5061/dryad.rc63v/1110.137 2023-06-13T13:21:04Z Sea ice is an analog environment for several of astrobiology’s near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to liquid water below in order to detect life. We have developed a submersible digital holographic microscope (DHM) that is capable of resolving individual bacterial cells, and demonstrated its utility for immediately imaging samples taken directly from sea ice at several locations near Nuuk, Greenland. In all samples, the appearance and motility of eukaryotes were conclusive signs of life. The appearance of prokaryotic cells alone was not sufficient to confirm life, but when prokaryotic motility occurred, it was rapid and conclusive. Warming the samples to above-freezing temperatures or supplementing with serine increased the number of motile cells and the speed of motility; supplementing with serine also stimulated chemotaxis. These results show that DHM is a useful technique for detection of active organisms in extreme environments, and that motility may be used as a biosignature in the liquid brines that persist in ice. These findings have important implications for the design of missions to icy environments and suggest ways in which DHM imaging may be integrated with chemical life-detection suites in order to create more conclusive life detection packages. Other/Unknown Material Greenland Nuuk Sea ice Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) Greenland Nuuk ENVELOPE(-52.150,-52.150,68.717,68.717) Nature 276 5683 53 55 |
institution |
Open Polar |
collection |
Data Archiving and Networked Services (DANS): EASY (KNAW - Koninklijke Nederlandse Akademie van Wetenschappen) |
op_collection_id |
ftdans |
language |
unknown |
topic |
Life sciences medicine and health care |
spellingShingle |
Life sciences medicine and health care Lindensmith, Christian A. Rider, Stephanie Bedrossian, Manuel Wallace, J. Kent Serabyn, Eugene Showalter, Gordon M. Deming, Jody W. Nadeau, Jay L. Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
topic_facet |
Life sciences medicine and health care |
description |
Sea ice is an analog environment for several of astrobiology’s near-term targets: Mars, Europa, Enceladus, and perhaps other Jovian or Saturnian moons. Microorganisms, both eukaryotic and prokaryotic, remain active within brine channels inside the ice, making it unnecessary to penetrate through to liquid water below in order to detect life. We have developed a submersible digital holographic microscope (DHM) that is capable of resolving individual bacterial cells, and demonstrated its utility for immediately imaging samples taken directly from sea ice at several locations near Nuuk, Greenland. In all samples, the appearance and motility of eukaryotes were conclusive signs of life. The appearance of prokaryotic cells alone was not sufficient to confirm life, but when prokaryotic motility occurred, it was rapid and conclusive. Warming the samples to above-freezing temperatures or supplementing with serine increased the number of motile cells and the speed of motility; supplementing with serine also stimulated chemotaxis. These results show that DHM is a useful technique for detection of active organisms in extreme environments, and that motility may be used as a biosignature in the liquid brines that persist in ice. These findings have important implications for the design of missions to icy environments and suggest ways in which DHM imaging may be integrated with chemical life-detection suites in order to create more conclusive life detection packages. |
author |
Lindensmith, Christian A. Rider, Stephanie Bedrossian, Manuel Wallace, J. Kent Serabyn, Eugene Showalter, Gordon M. Deming, Jody W. Nadeau, Jay L. |
author_facet |
Lindensmith, Christian A. Rider, Stephanie Bedrossian, Manuel Wallace, J. Kent Serabyn, Eugene Showalter, Gordon M. Deming, Jody W. Nadeau, Jay L. |
author_sort |
Lindensmith, Christian A. |
title |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_short |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_full |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_fullStr |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_full_unstemmed |
Data from: A submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
title_sort |
data from: a submersible, off-axis holographic microscope for detection of microbial motility and morphology in aqueous and icy environments |
publishDate |
2016 |
url |
http://nbn-resolving.org/urn:nbn:nl:ui:13-21-c6ja https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:92181 |
long_lat |
ENVELOPE(-52.150,-52.150,68.717,68.717) |
geographic |
Greenland Nuuk |
geographic_facet |
Greenland Nuuk |
genre |
Greenland Nuuk Sea ice |
genre_facet |
Greenland Nuuk Sea ice |
op_relation |
doi:10.5061/dryad.rc63v/1 doi:10.5061/dryad.rc63v/2 doi:10.5061/dryad.rc63v/3 doi:10.5061/dryad.rc63v/4 doi:10.5061/dryad.rc63v/5 doi:10.5061/dryad.rc63v/6 doi:10.5061/dryad.rc63v/7 doi:10.5061/dryad.rc63v/8 doi:10.5061/dryad.rc63v/9 doi:10.5061/dryad.rc63v/10 doi:10.5061/dryad.rc63v/11 doi:10.1371/journal.pone.0147700 PMID:26812683 http://nbn-resolving.org/urn:nbn:nl:ui:13-21-c6ja doi:10.5061/dryad.rc63v https://easy.dans.knaw.nl/ui/datasets/id/easy-dataset:92181 |
op_rights |
OPEN_ACCESS: The data are archived in Easy, they are accessible elsewhere through the DOI https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf |
op_doi |
https://doi.org/10.5061/dryad.rc63v/110.5061/dryad.rc63v/210.5061/dryad.rc63v/310.5061/dryad.rc63v/410.5061/dryad.rc63v/510.5061/dryad.rc63v/610.5061/dryad.rc63v/710.5061/dryad.rc63v/810.5061/dryad.rc63v/910.5061/dryad.rc63v/1010.5061/dryad.rc63v/1110.137 |
container_title |
Nature |
container_volume |
276 |
container_issue |
5683 |
container_start_page |
53 |
op_container_end_page |
55 |
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1770272024279646208 |